| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,341 | 0 | G | A | 45.0% | 14.6 / 12.3 | 20 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base G (3/8); new base A (9/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT > NZ_CP009273/2380212‑2380478 | cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGca < 1:430476/139‑1 (MQ=255) tACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACat < 1:586998/139‑1 (MQ=255) gCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCCAAAAAAAGACACGCGCaatttatttt > 2:391829/1‑139 (MQ=255) gCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattttatttt > 1:322855/1‑139 (MQ=255) tACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGAACTGCACATTTTTTTTTTCCTCATCt > 2:27020/1‑139 (MQ=255) aTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTATTTTTCCTCATCTGTTTTAAGATGc > 1:525492/1‑139 (MQ=255) aTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCaa < 2:322855/139‑1 (MQ=255) tAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGaaaa < 1:391829/139‑1 (MQ=255) tataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGGACATTTTTTTTTTCCTCATCTGTTTTTAGAAGCGAAAAATGAAAATTAAAGGAGGAAAAATAAAAGGGGAt > 1:62284/1‑138 (MQ=255) ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTATAGAAAAAAAACAAAGCCCACTTTATTTTTTCCCAACTGTTTTTTGATGCGAAAAATAAAAATCAAAGGGGGGAAAAAAAATTGGGATGATTGGCt > 2:323674/1‑139 (MQ=255) caGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAGATTCGCAACATTAAAATTAACGGAGGAAAAAAAAATGGGAATGATTGGGTAttttttaga > 2:415024/1‑133 (MQ=255) gTAAATAATAAAAATTTCTCGAGGCGGGGTCCGAAAAAAAGAAATGTACAATTTATTTTTTCTCATCTGTTTTTAGATGCGTAAAACTAAAATTAAAGGAGGGAAAAAAAATAGGAATGAATGGGTATTTTGCAGaaa > 1:190947/1‑138 (MQ=255) gTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAGCGACATGCACAATTTTTTTTTCCTCATCTGTTTTAAAATGGGAAACATGAAAATTAAAGGAGGGAAAAAAAATGGGAATGATTTGCTATTTTTCAGaaaa > 1:404816/1‑138 (MQ=255) ataataAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGAc < 2:380836/139‑1 (MQ=255) taataaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACt < 2:525492/139‑1 (MQ=255) aaaTTTCTCGAGGCGGGGCCAGAAAAAACGACATGGACATTTTTTTTTTCCTCCTCTTTTTTTAAATGCGAAAAATGAAAATCAACGGAGGAAAAAAAAATGGGAATGATTGGCTATTTTTCAGAAATTGACTCAGaa > 1:380836/1‑138 (MQ=255) gAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTaa < 2:27107/137‑1 (MQ=255) gccAAAAAAAACGACATGCACATTTTATTTTTCCTCATCTGTTTTAAAATGGGGAAAATGAAATTCCAAGGAGGAAAAATAAATTGGAATGATTTGCTATTTTGCAGAAAATGACTCAGAAAAAATTAAACAACTGCtt > 1:572812/2‑139 (MQ=255) cAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGa < 1:27020/139‑1 (MQ=255) aGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACt > 2:32089/1‑139 (MQ=255) | CAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT > NZ_CP009273/2380212‑2380478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |