Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,012,9510AT39.3% 36.1 / 18.6 28L144F (TTA→TTT) ssnAputative aminohydrolase SsnA
Reads supporting (aligned to +/- strand):  ref base A (7/10);  new base T (11/0);  total (18/10)
Fisher's exact test for biased strand distribution p-value = 1.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGAGTCGCTCTATTACAGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGAC  >  NZ_CP009273/3012819‑3013079
                                                                                                                                      |                                                                                                                                
agagTCGCTCTATTACAGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGtt                                                                                                                            <  2:523410/139‑1 (MQ=255)
   gTCGCTCTATTACAGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGTCCTCTCCCCATTTCGC‑GACC‑CATCTTTATAAGTTggg                                                                                                                         >  2:85231/1‑138 (MQ=255)
                  cGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGAcc                                                                                                          <  1:404989/139‑1 (MQ=255)
                    gACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGGTCCCCCCCATGCGC‑GGCG‑CCTTTTTTTTAAATGGCGCTCGCGCGGTGACCTg                                                                                                        >  2:404989/1‑139 (MQ=255)
                    gACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCACACTTCGCGGCCC‑CTTTTTTTAAAGTT‑GCCTTCGCGCGCTGGCCTg                                                                                                        >  2:659485/1‑139 (MQ=255)
                    gACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCCCTCCACCTTTCGC‑GCCG‑CATTTTTTTAAATTGGGCGGCGCGCGCGGACCtc                                                                                                        >  1:431542/1‑138 (MQ=255)
                          tttGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGa                                                                                                  <  1:659485/139‑1 (MQ=255)
                              ttCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCCCCCTGCGC‑GGCG‑CCTTTTTTTAAATTTGCCTGCGCGCGCTGCCCTGtttttttac                                                                                               >  2:557743/1‑133 (MQ=255)
                                     gAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGAcc                                                                                       <  1:274687/139‑1 (MQ=255)
                                                 agCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCCCTCCCCCATTCGC‑GGCGCCCTTTTTTAAAGTGGGCC‑GCGCGCGGGGGCCCGTTTTTGAACTACTTGCCCTaacaaaagc                                                                            >  2:10511/1‑134 (MQ=255)
                                                     gATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGGTCCTCTCCCTTCGC‑GCCG‑CCTTTTTTTAAATTTGCCTGCCCCCGCTGTACTCTTTTGTAACGACTTAACCTACCAACGCCagcc                                                                        >  2:176594/1‑135 (MQ=255)
                                                      aTGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCaaa                                                                      <  2:613380/139‑1 (MQ=255)
                                                                gTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAgg                                                            <  1:557743/139‑1 (MQ=255)
                                                                 ttATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCCCCTTTCGC‑GACG‑CCTTTTTTTAAATTTGCCCTCGCGCGCTGGCCCCCTTTTTAACAAATGTCCGCAACAACCGCAACAAAAAGTTGGAgg                                                            >  2:364682/1‑138 (MQ=255)
                                                                    tCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCGCTTCACATTGACC‑GCCG‑CCCTTTTTTAAGATGGCCTGCGCGCGGTGCCCTGTTTTTTAAATACTATTCGTAAAAAACGCATCACAGAGGTGTCGGAAgg                                                        >  2:258878/1‑139 (MQ=255)
                                                                           ccATGCCTCTCCGGCGTATATCGGCGGGGCGGTCTCCCCATTCCGC‑GCCG‑CCTTTTTTAAAATTGGCCTGCGCGCGGTGAACTGGTTTTAAACTACTTTCCGGAACAACAGCATCAAAGAGATGCAGGAAgggggag                                                   >  2:189695/1‑132 (MQ=255)
                                                                              tGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAgaagaa                                              <  1:210450/139‑1 (MQ=255)
                                                                              tGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAgaagaa                                              <  1:75553/139‑1 (MQ=255)
                                                                              tGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCACAATGCGC‑GAAG‑CACTTTTTTAAGTTGGGCTGCGCGCGGGGAGCTGTTTTTAAAATAATGCACGGAAAAACCGCAACCAAAAGGGGGAGGAAGGGGGggaaga                                               >  1:517354/1‑138 (MQ=255)
                                                                                   ctcCGGCGTATATCGGCGGGGAGCTCTCCACACTGCGC‑GGCG‑CAATTTTTAAAAATGGGCTGGGCGCGGGGAACGGGTTTGAAACTACTGACCGTGACAACGGCATCAAAAAGTTGCAGGAAGGAGTGGAAGAAAACaa                                         >  1:573418/1‑138 (MQ=255)
                                                                                                         gCTCACCACATTGCGA‑TACG‑CATTTTTAAAAACGGACATGCGCGCGATGACCTGTTATGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAATACATCCGTTTCGCCCGTTtgattgat                   <  2:89121/139‑1 (MQ=255)
                                                                                                                    tGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCgaaga         >  1:632322/1‑138 (MQ=255)
                                                                                                                    tGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCgaaga         >  1:43152/1‑138 (MQ=255)
                                                                                                                    tGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCgaaga         >  1:225593/1‑138 (MQ=255)
                                                                                                                       gc‑gACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGc     >  1:413661/1‑139 (MQ=255)
                                                                                                                          gACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGa   <  2:431542/139‑1 (MQ=255)
                                                                                                                          gACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGAAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGa   >  1:64562/1‑139 (MQ=255)
                                                                                                                            cG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGAc  >  1:408797/1‑138 (MQ=255)
                                                                                                                                      |                                                                                                                                
AGAGTCGCTCTATTACAGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGC‑GACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGAC  >  NZ_CP009273/3012819‑3013079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑