Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,502,0160AG43.3% 40.3 / 10.2 30F386S (TTC→TCC) yhfWphosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (1/16);  new base G (13/0);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 1.17e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGT  >  NZ_CP009273/3501889‑3502143
                                                                                                                               |                                                                                                                               
cGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATcaca                                                                                                                       >  1:632984/1‑138 (MQ=255)
         cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACAAACCGTAAcc                                                                                                             >  1:1781/1‑139 (MQ=255)
                 aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGCGGGAAAAATAACAAAAGGGAGACCCcacct                                                                                                      >  1:212917/1‑136 (MQ=255)
                    tGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAg                                                                                                   >  1:109322/1‑138 (MQ=255)
                      cTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGGAGCCCCCCCAACAgca                                                                                                 >  2:80267/1‑136 (MQ=255)
                        cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACAAACGGGAGCCCCCCCACCAGaaa                                                                                                >  2:133462/1‑137 (MQ=255)
                          tGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgt                                                                                            <  2:113944/139‑1 (MQ=255)
                                      tAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGTAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGTGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACAcc                                                                                <  2:47697/139‑1 (MQ=255)
                                      tAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACAcc                                                                                <  1:570513/139‑1 (MQ=255)
                                         cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAg                                                                             <  1:284850/139‑1 (MQ=255)
                                         cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAg                                                                             <  2:330543/139‑1 (MQ=255)
                                         cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAg                                                                             <  2:318327/139‑1 (MQ=255)
                                         cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAg                                                                             <  2:285811/139‑1 (MQ=255)
                                              cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGGAGCCCCCACATCAGAAAGCGGGGGGCGCACAACGAGCTGCg                                                                        >  1:257956/1‑139 (MQ=255)
                                              cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACAGGAGCCCCCCCATCAGAAAACGTGGTGCGCACACCCCGCTGCg                                                                        >  2:570513/1‑139 (MQ=255)
                                              cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATAAAACACGGTAACCCCCCCATCCGAAAACGGGGGGGGGACACCCAGCTGCg                                                                        >  2:284850/1‑139 (MQ=255)
                                              cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTAGAGCGCGGGAAAAATAAAAAACCGGGACCCCCCCAACAGAAAGGGGGGTGGGCACACCGAGCTGCg                                                                        >  2:199566/1‑139 (MQ=255)
                                               ggATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCg                                                                        <  2:109322/138‑1 (MQ=255)
                                                gATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTa                                                                      <  1:220321/139‑1 (MQ=255)
                                                                   tctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGGAGCCCCCCCAACAGAAAGCGGGGGGGGCACAACGAGCTGCGGGGCGGTCAACCCCTTCTgg                                                    >  2:4670/1‑137 (MQ=255)
                                                                          cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAcc                                              <  2:124736/137‑1 (MQ=255)
                                                                            aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCa                                          <  1:80267/139‑1 (MQ=255)
                                                                             aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAc                                         <  1:199566/139‑1 (MQ=255)
                                                                               cGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTCACACACGGGAGCCCCCACAACAGAAAGCGGGGGGCGGACACCCAGCCGCGCAGCGCTCATCCCCTTCTGATAAAACCGCACTg                                       >  2:202792/1‑139 (MQ=255)
                                                                                         aaagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACCCGGGAACCCCCCCATCCGAAAACGGGGGGCGCACACCCAGCTGCGTAGCGATCCTCCCCTTCTTATAAACCAGCCCCGGCCCCCCTTc                             >  2:655887/1‑139 (MQ=255)
                                                                                         aaagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                             <  1:599123/139‑1 (MQ=255)
                                                                                                agagCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACCGTAGCCCCCCCATCAGAAAGCGGGGGGCGCACACCCAGCTGCCTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTa                      >  1:27084/1‑139 (MQ=255)
                                                                                                        cATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCt              <  2:313675/139‑1 (MQ=255)
                                                                                                              gtggcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGAc         <  1:202792/138‑1 (MQ=255)
                                                                                                                    gtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGt  <  1:133462/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                               
CGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGT  >  NZ_CP009273/3501889‑3502143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑