| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,612,067 | 0 | A | G | 58.8% | 1.6 / 15.1 | 17 | E161G (GAG→GGG) | yjjJ | type II toxin‑antitoxin system HipA family toxin YjjJ |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (10/0); total (13/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGT > NZ_CP009273/4611930‑4612178 | tttAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCgggga < 1:90829/138‑1 (MQ=255) aaGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTa < 2:14628/139‑1 (MQ=255) tAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGGATTATCAGCGGTGGATTACttg > 2:86154/1‑137 (MQ=255) cgcAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTTGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCacaaca > 2:633143/1‑139 (MQ=255) cgcAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGAATTATTAGCGATGGATTACTGCacaaca > 2:318475/1‑139 (MQ=255) gactgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTTGTTTGTCGGGGGGGGGAATTATCAGCGAGGGATTACTTCACCACCCCCCGCGGGAAtt > 2:522277/1‑139 (MQ=255) taCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTTGTTGGTCGGGGGGGGGAATTATCAGCGGTGGGTTACTGCCCAACAACCTGCGGAAATTTCTCTGGATc > 2:117034/1‑138 (MQ=255) taCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATCTAACGGTGAATATACTGGCGGTTGGTTGGTCggggaggg > 2:165921/1‑85 (MQ=255) taCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATCTAACGGTGAATATACTGGCGGTTGGTTGGTCggggaggg < 1:165921/85‑1 (MQ=255) cGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTTGTCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAAATCCTCTGGGTCaa > 2:52670/1‑138 (MQ=255) ctctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTTGATCAAAAAc > 2:296057/1‑139 (MQ=255) ggAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAAAACCCTGCGGAAATTCCTCTGGATCAAAAAATCACCCaa > 1:545958/1‑138 (MQ=255) cGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAg < 1:117034/138‑1 (MQ=255) aCCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCa < 1:516782/138‑1 (MQ=255) gTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTa > 2:508299/1‑64 (MQ=255) gTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTa < 1:508299/64‑1 (MQ=255) aCGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGGATTATTAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGGTCCAAAACTCACCCATTACGAACAGCTGGCAAGGGATGCACTGGCAGgag > 2:154647/1‑138 (MQ=255) tGAATATACTGGCGGTTGGGTTGTCGGGGGGGGGAATTATCAGCGATGGGTTACTGCCCAACACCCTGCGGGAAATCCTCTGGGTCAAAAAATCACCCATTTCGAACAGCTGGCAAAGGGTGTCCTTGCAGGAGAAAtt > 2:585346/1‑139 (MQ=255) tGAATATACTGGCGGATGGTTGGGCGGGGAGGGGGATTATAAGGGATGGGATACGGGGCAAAACCCCGCGGAAATTCCTCTGGAGCAAAAACTAACCCATTACAAACAGCTGGCAAGTGATGCACTGGCAGGAGaaaat > 2:391786/1‑137 (MQ=255) aaTATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGGAATTCCTCTGGGTCAAAAAATCACCCCTTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATtgt > 1:511781/1‑139 (MQ=255) | TTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGT > NZ_CP009273/4611930‑4612178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |