Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→G	W30G (TGG→GGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	G	100.0%	85.6 / NA	24	W30G (TGG→GGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/12);  total (12/12)

ACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACG  >  NZ_CP009273/49784‑50030                                                                                                                               |                                                                                                                         aCGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGc                                                                                                               >  1:535467/1‑138 (MQ=255)      taGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa                                                                                                             >  2:452564/1‑135 (MQ=255)      taGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa                                                                                                             <  1:452564/135‑1 (MQ=255)      taGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACAcc                                                                                                         <  2:78649/139‑1 (MQ=255)            cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTtaaa                                                                                                    >  2:315753/1‑138 (MQ=255)                                  aTCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAATCCCGTGATTATGGgccgcc                                                                             <  1:430823/139‑1 (MQ=255)                                       aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                                                                            <  2:535467/135‑1 (MQ=255)                                           aTCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg                                                                    <  1:166638/139‑1 (MQ=255)                                             cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaa                                                                  >  2:309891/1‑139 (MQ=255)                                                     ttGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                                                  >  1:180646/1‑115 (MQ=255)                                                     ttGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                                                  <  2:180646/115‑1 (MQ=255)                                                        cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcg                                                       >  1:479570/1‑139 (MQ=255)                                                          gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTtaaat                                                                                                   <  2:510109/93‑1 (MQ=255)                                                          gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTtaaat                                                                                                   >  1:510109/1‑93 (MQ=255)                                                                      gATCGCGTTATCGGCAGGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGa                                          <  2:330891/138‑1 (MQ=255)                                                                          gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                     <  1:309891/139‑1 (MQ=255)                                                                           cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                     >  2:324987/1‑138 (MQ=255)                                                                           cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCAGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                     >  2:37792/1‑138 (MQ=255)                                                                                                  tGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAAcc             >  1:207094/1‑139 (MQ=255)                                                                                                    ccGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCAGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCgg           <  1:37792/139‑1 (MQ=255)                                                                                                       tGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                                                                 <  2:155997/66‑1 (MQ=255)                                                                                                       tGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                                                                 >  1:155997/1‑66 (MQ=255)                                                                                                       tGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTa        >  1:483984/1‑139 (MQ=255)                                                                                                             ctgcctgcCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACg  <  1:234880/139‑1 (MQ=255)                                                                                                                               |                                                                                                                         ACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACG  >  NZ_CP009273/49784‑50030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑