Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,745,340	+G	coding (633/639 nt)	ydhW ←	YdhW family putative oxidoreductase system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,745,336	1	.	G	100.0%	160.6 / NA	43	*213R (TGA→CGA)	ydhW	YdhW family putative oxidoreductase system protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (29/14);  total (29/14)

GAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCA‑GGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAA  >  NZ_CP009273/1745258‑1745417                                                                                |                                                                                  gAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGaa                                                                        >  2:490496/1‑91 (MQ=255) gAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGaa                                                                        >  2:853833/1‑91 (MQ=255)        ggACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGctc                                                                 <  1:185345/91‑1 (MQ=255)        ggACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGctc                                                                 <  1:408853/91‑1 (MQ=255)        ggACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGctc                                                                 <  1:604103/91‑1 (MQ=255)             tCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCt                                                            >  1:722346/1‑91 (MQ=255)                     tCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCAt                                                    >  1:800275/1‑91 (MQ=255)                        ccTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCg                                                 >  1:332278/1‑91 (MQ=255)                          tAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGca                                               >  2:918589/1‑91 (MQ=255)                                aGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGcataagcc                                         >  1:1116717/1‑91 (MQ=255)                                 gTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGcataagcca                                        >  2:777911/1‑91 (MQ=255)                                 gTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGcataagcca                                        >  2:986748/1‑91 (MQ=255)                                 gTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCA‑ggg                                                                                <  1:868260/50‑1 (MQ=255)                                 gTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCA‑ggg                                                                                >  2:868260/1‑50 (MQ=255)                                       gCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCataagccataagcc                                  <  2:938063/91‑1 (MQ=255)                                       gCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCataagccataagcc                                  <  2:942919/91‑1 (MQ=255)                                       gCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCataagccataagcc                                  <  1:258542/91‑1 (MQ=255)                                             gTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCa                            >  2:232298/1‑91 (MQ=255)                                             gTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCa                            >  2:640132/1‑91 (MQ=255)                                             gTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCa                            >  2:245393/1‑91 (MQ=255)                                             gTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCa                            >  1:934032/1‑91 (MQ=255)                                               ccACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATa                          <  1:890964/91‑1 (MQ=255)                                               ccACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATa                          >  2:140602/1‑91 (MQ=255)                                               ccACCCTATCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATa                          <  2:455476/91‑1 (MQ=255)                                                      aTCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATa                          >  2:230880/1‑84 (MQ=255)                                                      aTCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATa                          <  1:230880/84‑1 (MQ=255)                                                       tCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATaa                  >  2:35014/1‑91 (MQ=255)                                                       tCCATGACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATaa                  >  2:718791/1‑91 (MQ=255)                                                            gACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCt                                 >  2:78187/1‑71 (MQ=255)                                                            gACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCt                                 <  1:78187/71‑1 (MQ=255)                                                            gACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgc             >  2:405490/1‑91 (MQ=255)                                                            gACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgc             >  2:448376/1‑91 (MQ=255)                                                            gACATCTCCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgc             >  2:600885/1‑91 (MQ=255)                                                                tctcCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctcgc         >  2:993856/1‑91 (MQ=255)                                                                tctcCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctcgc         >  1:984042/1‑91 (MQ=255)                                                                tctcCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctcgc         >  1:905274/1‑91 (MQ=255)                                                                tctcCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctcgc         >  1:699415/1‑91 (MQ=255)                                                                tctcCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctcgc         >  1:257693/1‑91 (MQ=255)                                                                tctcCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctcgc         >  1:1106570/1‑91 (MQ=255)                                                                 ctcCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATcgctcgct        <  1:875783/91‑1 (MQ=255)                                                                 ctcCCTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATcgctcgct        <  1:5969/91‑1 (MQ=255)                                                                   cccTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTaa      >  2:625421/1‑91 (MQ=255)                                                                    ccTGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTaaa     <  1:640132/91‑1 (MQ=255)                                                                      tGAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACa   <  1:352128/91‑1 (MQ=255)                                                                       gAAATTTCAGGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCATTGCCATAGGTCATAAATCGCTCGCTAAACaa  >  1:1055279/1‑91 (MQ=255)                                                                                |                                                                                  GAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCA‑GGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAA  >  NZ_CP009273/1745258‑1745417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑