Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,292,953	+C	coding (322/444 nt)	yhbP ←	YhbP family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,292,952	1	.	C	100.0%	72.0 / NA	21	A108G (GCC→GGC)	yhbP	YhbP family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (3/18);  total (3/18)

TTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGG‑CAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTT  >  NZ_CP009273/3292871‑3293027                                                                                   |                                                                            tttttACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGa                                                                     <  1:665046/91‑1 (MQ=255) tttttACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGa                                                                     <  1:95156/91‑1 (MQ=255)        cAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGcgac                                                              <  1:443839/91‑1 (MQ=255)         aaaGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGcgacg                                                             <  2:627624/91‑1 (MQ=255)          aaGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGcgacga                                                            <  1:518477/91‑1 (MQ=255)                              gAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAg                                        >  2:757964/1‑91 (MQ=255)                                        tCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTcc                              >  2:1105563/1‑91 (MQ=255)                                          aTCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTc                            <  1:686380/91‑1 (MQ=255)                                           tCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCa                           <  1:1110729/91‑1 (MQ=255)                                           tCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCa                           <  2:867489/91‑1 (MQ=255)                                                       tCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTg               <  1:984581/91‑1 (MQ=255)                                                               cGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtctc       <  2:145220/91‑1 (MQ=255)                                                               cGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtctc       <  1:546495/91‑1 (MQ=255)                                                               cGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtctc       <  2:81471/91‑1 (MQ=255)                                                               cGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtctc       <  1:1105563/91‑1 (MQ=255)                                                                ggggCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCa      >  2:336553/1‑91 (MQ=255)                                                                    ccGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCtt  <  1:957090/91‑1 (MQ=255)                                                                    ccGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCtt  <  2:1062622/91‑1 (MQ=255)                                                                    ccGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCtt  <  1:757964/91‑1 (MQ=255)                                                                    ccGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCtt  <  2:588883/91‑1 (MQ=255)                                                                    ccGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCtt  <  1:550557/91‑1 (MQ=255)                                                                                   |                                                                            TTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGG‑CAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTT  >  NZ_CP009273/3292871‑3293027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑