Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,765,154	A→G	N95D (AAT→GAT)	ydiL →	YdiL family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,765,154	0	A	G	100.0%	134.6 / NA	37	N95D (AAT→GAT)	ydiL	YdiL family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/13);  total (24/13)

AAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAA  >  NZ_CP009273/1765074‑1765241                                                                                 |                                                                                        aaataaatAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGa                                                                               >  2:1068946/1‑91 (MQ=255) aaataaatAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGa                                                                               >  1:889731/1‑91 (MQ=255) aaataaatAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGa                                                                               >  1:471396/1‑91 (MQ=255)       ataaCCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGaa                                                                         <  1:610529/91‑1 (MQ=255)             gCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTa                                                                   >  1:851636/1‑91 (MQ=255)               aTCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATc                                                                 <  2:630108/91‑1 (MQ=255)               aTCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATc                                                                 <  2:301299/91‑1 (MQ=255)                            ccATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgctgc                                                    >  2:538727/1‑91 (MQ=255)                            ccATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgctgc                                                    >  2:444364/1‑91 (MQ=255)                            ccATGCGGTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgctgc                                                    >  1:1044914/1‑91 (MQ=255)                             cATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTg                                                                                >  2:1005793/1‑62 (MQ=255)                             cATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTg                                                                                <  1:1005793/62‑1 (MQ=255)                             cATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgctgct                                                   >  1:363502/1‑91 (MQ=255)                             cATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgctgct                                                   >  2:495412/1‑91 (MQ=255)                              aTGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGctgctg                                                  >  1:542800/1‑91 (MQ=255)                                gCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTa                                                         <  2:148285/82‑1 (MQ=255)                                gCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTa                                                         >  1:148285/1‑82 (MQ=255)                                    tttttCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATtat                                            >  2:76597/1‑91 (MQ=255)                                            cGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    <  2:990218/91‑1 (MQ=255)                                            cGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    <  2:851636/91‑1 (MQ=255)                                                      gCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATAAGCACACGATCTGGAGc                          <  1:368535/91‑1 (MQ=255)                                                       cATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCg                         <  2:869537/91‑1 (MQ=255)                                                        aTTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCgg                        >  1:390547/1‑91 (MQ=255)                                                                 aGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTaa               >  2:341757/1‑91 (MQ=255)                                                                   tCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTa                                                         >  1:134491/1‑47 (MQ=255)                                                                   tCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTa                                                         <  2:134491/47‑1 (MQ=255)                                                                   tCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAAtt             >  2:394390/1‑91 (MQ=255)                                                                   tCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAAtt             >  1:779195/1‑91 (MQ=255)                                                                           gATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    >  2:150709/1‑60 (MQ=255)                                                                           gATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    <  1:150709/60‑1 (MQ=255)                                                                           gATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    >  1:877981/1‑60 (MQ=255)                                                                           gATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    <  2:877981/60‑1 (MQ=255)                                                                           gATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCcaca     >  2:381853/1‑91 (MQ=255)                                                                           gATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCcaca     >  2:510414/1‑91 (MQ=255)                                                                           gATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCcaca     >  1:1080988/1‑91 (MQ=255)                                                                           gATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCcaca     >  2:1042398/1‑91 (MQ=255)                                                                              ggCGATTTTATTGGCTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGaa  <  2:74944/91‑1 (MQ=255)                                                                                 |                                                                                        AAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAA  >  NZ_CP009273/1765074‑1765241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑