Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,955,016	A→G	D233G (GAC→GGC)	argS →	arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,955,016	0	A	G	100.0%	112.0 / NA	31	D233G (GAC→GGC)	argS	arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/11);  total (20/11)

AGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACA  >  NZ_CP009273/1954932‑1955102                                                                                     |                                                                                       agTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCAc                                                                                  >  2:280471/1‑91 (MQ=255) agTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCAc                                                                                  >  1:635329/1‑91 (MQ=255) agTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCAc                                                                                  >  2:23889/1‑91 (MQ=255) agTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCAc                                                                                  >  2:131741/1‑91 (MQ=255)    tCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCAt                                                                               >  1:1026838/1‑91 (MQ=255)    tCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCAt                                                                               >  2:882302/1‑91 (MQ=255)    tCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCAt                                                                               >  2:423127/1‑91 (MQ=255)         gAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGc                                                                          <  1:357586/91‑1 (MQ=255)         gAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGc                                                                          <  2:1052166/91‑1 (MQ=255)             gcgcACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGaa                                                                      <  2:916466/91‑1 (MQ=255)                          cGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTAc                                                         >  2:970345/1‑91 (MQ=255)                            tGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGa                                                       >  1:145043/1‑91 (MQ=255)                            tGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGa                                                       >  2:1114933/1‑91 (MQ=255)                            tGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGa                                                       >  2:855840/1‑91 (MQ=255)                             tggtaaaCTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGAt                                                      <  1:451432/87‑1 (MQ=255)                              gTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATc                                                     >  1:782768/1‑91 (MQ=255)                              gTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATc                                                     <  1:649080/91‑1 (MQ=255)                              gTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATc                                                     >  2:649080/1‑91 (MQ=255)                                      gCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAAc                                             >  2:557280/1‑91 (MQ=255)                                      gCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAAc                                             >  2:475437/1‑91 (MQ=255)                                        aaaGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGt                                           <  2:252103/91‑1 (MQ=255)                                          aGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGa                                         <  1:323244/91‑1 (MQ=255)                                               tGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTg                                    <  2:407414/91‑1 (MQ=255)                                                        tttCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGtgat                           >  1:526805/1‑91 (MQ=255)                                                        tttCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGtgat                           >  2:772805/1‑91 (MQ=255)                                                              cgAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTg                     >  2:1045350/1‑91 (MQ=255)                                                                 gaTGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATg                  <  1:334598/91‑1 (MQ=255)                                                                      ggCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTTCGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGa             <  1:882302/91‑1 (MQ=255)                                                                      ggCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGa             <  2:656914/91‑1 (MQ=255)                                                                            aaCTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCct       >  2:819632/1‑91 (MQ=255)                                                                                 gTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACa  >  2:568851/1‑91 (MQ=255)                                                                                     |                                                                                       AGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACA  >  NZ_CP009273/1954932‑1955102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑