Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	251,113	A→G	I364T (ATC→ACC)	pepD ←	cytosol nonspecific dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	251,113	0	A	G	100.0%	111.2 / NA	30	I364T (ATC→ACC)	pepD	cytosol nonspecific dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/12);  total (18/12)

TTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGG  >  NZ_CP009273/251036‑251200                                                                              |                                                                                        ttttCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAAt                                                                            <  1:1092312/91‑1 (MQ=255)    tCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTc                                                                         <  2:1097542/91‑1 (MQ=255)           agccagTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTa                                                                  <  1:174944/91‑1 (MQ=255)           agccagTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTa                                                                  <  1:617803/91‑1 (MQ=255)                gTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTgtc                                                             <  2:1114754/91‑1 (MQ=255)                        aGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATgg                                                     >  2:992989/1‑91 (MQ=255)                         gCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCACGGt                                                    >  1:769185/1‑91 (MQ=255)                          cGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTc                                                   >  2:992225/1‑91 (MQ=255)                          cGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTc                                                   <  2:555726/91‑1 (MQ=255)                                   cATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCg                                          <  1:131179/91‑1 (MQ=255)                                           ccacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAg                                  >  2:564307/1‑91 (MQ=255)                                            cacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAgg                                 >  1:996232/1‑91 (MQ=255)                                                tAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAgg                             >  1:840677/1‑91 (MQ=255)                                                tAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAgg                             >  1:400604/1‑91 (MQ=255)                                                  gTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGtt                           <  2:885915/91‑1 (MQ=255)                                                  gTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGtt                           >  2:311078/1‑91 (MQ=255)                                                   tCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGttt                          >  1:941838/1‑91 (MQ=255)                                                     tttACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCa                        >  1:296382/1‑91 (MQ=255)                                                     tttACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCa                        >  1:384284/1‑91 (MQ=255)                                                     tttACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCa                        >  2:417291/1‑91 (MQ=255)                                                          cGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACcac                   <  2:391560/91‑1 (MQ=255)                                                            cTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCacac                 >  1:254689/1‑91 (MQ=255)                                                            cTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCacac                 >  1:802313/1‑91 (MQ=255)                                                            cTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCacac                 >  1:315699/1‑91 (MQ=255)                                                            cTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCacac                 >  2:294646/1‑91 (MQ=255)                                                            cTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCacac                 <  1:992225/91‑1 (MQ=255)                                                            cTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCacac                 >  1:973880/1‑91 (MQ=255)                                                                      gTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTAc       >  2:1117041/1‑91 (MQ=255)                                                                         aaCGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATc    <  2:404349/91‑1 (MQ=255)                                                                           cGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCgg  <  1:868028/91‑1 (MQ=255)                                                                              |                                                                                        TTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGG  >  NZ_CP009273/251036‑251200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑