| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,036,314 | A→G | F65S (TTC→TCC) | yqfA ← | hemolysin III family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,036,314 | 0 | A | G | 97.3% | 131.2 / NA | 37 | F65S (TTC→TCC) | yqfA | hemolysin III family protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (11/25); total (12/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCA > NZ_CP009273/3036227‑3036395 | aaCAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAca < 1:598762/91‑1 (MQ=255)aaCAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAca < 1:583517/91‑1 (MQ=255) cAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAcagc < 1:643620/91‑1 (MQ=255) cAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAcagc < 1:286184/91‑1 (MQ=255) cAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAcagc < 2:1022657/91‑1 (MQ=255) ggTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAcagcag > 2:507190/1‑91 (MQ=255) ggTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAcagcag < 2:724709/91‑1 (MQ=255) tAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGa < 1:460652/91‑1 (MQ=255) gcgcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATAc > 1:155748/1‑91 (MQ=255) cgcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACt < 1:984916/91‑1 (MQ=255) gcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTg > 2:1095389/1‑91 (MQ=255) gcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAACAGCAGGATCATACTg > 1:1024367/1‑91 (MQ=255) cAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTgc < 2:330592/91‑1 (MQ=255) aTGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTgccg > 1:413966/1‑91 (MQ=255) aTGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTgccg < 2:441476/91‑1 (MQ=255) aTTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATagag < 1:53305/91‑1 (MQ=255) tttGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCg > 1:885566/1‑91 (MQ=255) gCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCtg < 2:868877/91‑1 (MQ=255) cccGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCtgt < 2:48154/91‑1 (MQ=255) tgatgaGGAATGGCTTGATAGAGCGTCGAAGGGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcgc < 2:393295/91‑1 (MQ=255) tgatgaGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcgc < 2:68620/91‑1 (MQ=255) tgatgaGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcgc < 1:30439/91‑1 (MQ=255) tgatgaGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcgc < 2:918146/91‑1 (MQ=255) aaTGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTa < 1:955647/91‑1 (MQ=255) aTGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTaa > 1:72249/1‑91 (MQ=255) aTGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTaa > 2:239318/1‑91 (MQ=255) ggCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGa > 1:177431/1‑91 (MQ=255) gTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATcc < 1:272101/91‑1 (MQ=255) gATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc > 1:600899/1‑91 (MQ=255) aTAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAcc > 1:934617/1‑91 (MQ=255) gagCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGcc > 2:1054023/1‑91 (MQ=255) gagCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGcc < 1:1069412/91‑1 (MQ=255) agCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCt < 2:155748/91‑1 (MQ=255) agCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCt < 2:661131/91‑1 (MQ=255) gCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTg < 2:606172/91‑1 (MQ=255) gTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGaa < 1:529337/91‑1 (MQ=255) gAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCa < 1:311028/91‑1 (MQ=255) | AACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCA > NZ_CP009273/3036227‑3036395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |