Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,184,461	T→C	F157F (TTT→TTC)	yqiI →	fimbrial protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,184,461	0	T	C	100.0%	212.8 / NA	56	F157F (TTT→TTC)	yqiI	fimbrial protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/33);  total (23/33)

ATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAG  >  NZ_CP009273/3184373‑3184546                                                                                         |                                                                                      atatAAAGGGAGCCGGAACCTTCGGGAATGGCTGCAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTccc                                                                                     >  2:778261/1‑91 (MQ=255) atatAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTccc                                                                                     >  2:897185/1‑91 (MQ=255) atatAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTccc                                                                                     <  1:832520/91‑1 (MQ=255) atatAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTccc                                                                                     <  2:1073364/91‑1 (MQ=255) atatAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTccc                                                                                     >  1:673501/1‑91 (MQ=255) atatAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTccc                                                                                     >  1:191495/1‑91 (MQ=255)  tataAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCa                                                                                    <  2:447382/91‑1 (MQ=255)   ataAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAg                                                                                   >  2:869589/1‑91 (MQ=255)          gAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGt                                                                            <  1:839888/91‑1 (MQ=255)          gAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGt                                                                            >  1:993200/1‑91 (MQ=255)            gCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          <  2:620644/91‑1 (MQ=255)            gCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          <  2:399384/91‑1 (MQ=255)            gCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          <  2:416860/91‑1 (MQ=255)            gCCGGAACCTTCGGGAATGGATACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          <  2:393930/91‑1 (MQ=255)               ggAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          <  1:584847/88‑1 (MQ=255)               ggAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          >  2:584847/1‑88 (MQ=255)                gAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          <  2:307438/87‑1 (MQ=255)                gAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          >  1:307438/1‑87 (MQ=255)                gAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCt                                                                      >  1:483455/1‑91 (MQ=255)                gAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCt                                                                      <  2:103608/91‑1 (MQ=255)                 aaCCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     <  2:68366/91‑1 (MQ=255)                 aaCCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     <  2:111763/91‑1 (MQ=255)                 aaCCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     <  1:1039202/91‑1 (MQ=255)                      tCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     <  1:820600/86‑1 (MQ=255)                      tCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     >  2:820600/1‑86 (MQ=255)                          gAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAag                                                            <  1:1011107/91‑1 (MQ=255)                           aaTGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAagc                                                           <  2:473510/91‑1 (MQ=255)                           aaTGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAagc                                                           <  2:2438/91‑1 (MQ=255)                              ggCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     >  1:1123023/1‑78 (MQ=255)                              ggCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     <  2:1123023/78‑1 (MQ=255)                                      aaaGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAAt                                                <  2:677086/91‑1 (MQ=255)                                       aaGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATg                                               <  2:993200/91‑1 (MQ=255)                                                       gTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     >  1:587988/1‑53 (MQ=255)                                                       gTGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     <  2:587988/53‑1 (MQ=255)                                                        tGGCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGtaaataaa                              >  2:105027/1‑91 (MQ=255)                                                         ggCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCT‑AAAATGCCAATAAAGTAAATaaaaa                            >  2:965870/1‑91 (MQ=255)                                                          gCGGTCCTGAACCGCAATTATCAGTAACTTTCCCGGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATaaaaa                            >  1:607992/1‑91 (MQ=255)                                                          gCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAagct                                                          <  1:1000868/61‑1 (MQ=255)                                                          gCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAagct                                                          >  2:1000868/1‑61 (MQ=255)                                                          gCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATa                                         >  1:563879/1‑78 (MQ=255)                                                          gCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATa                                         <  2:563879/78‑1 (MQ=255)                                                          gCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATaaaaa                            >  2:990238/1‑91 (MQ=255)                                                          gCGGTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATaaaaa                            >  2:687569/1‑91 (MQ=255)                                                            ggTCGTGGACCGCATTTATCAGTAACTTTCCCCGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCCATAAAGTAAATAAAAAAc                          >  2:829459/1‑91 (MQ=255)                                                            ggTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     <  1:828051/48‑1 (MQ=255)                                                            ggTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCta                                                                     >  2:828051/1‑48 (MQ=255)                                                            ggTCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAAc                          >  1:905877/1‑91 (MQ=255)                                                              tCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          >  1:116075/1‑41 (MQ=255)                                                              tCCTGAACCGCAATTATCAGTAACTTTCCCAGTACGAGTGc                                                                          <  2:116075/41‑1 (MQ=255)                                                                      cGCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTAcc                <  1:160356/91‑1 (MQ=255)                                                                       gCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCt               >  1:663697/1‑91 (MQ=255)                                                                       gCAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCt               >  2:1011977/1‑91 (MQ=255)                                                                        cAATTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTg              <  1:702177/91‑1 (MQ=255)                                                                          aTTATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGAc            <  1:687569/91‑1 (MQ=255)                                                                           ttATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACg           <  1:491097/91‑1 (MQ=255)                                                                           ttATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACg           <  2:514104/91‑1 (MQ=255)                                                                           ttATCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACg           <  2:959731/91‑1 (MQ=255)                                                                             aTCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGaa         <  1:758948/91‑1 (MQ=255)                                                                             aTCAGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGaa         <  1:740556/91‑1 (MQ=255)                                                                                aGTAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAatat      >  2:1038306/1‑91 (MQ=255)                                                                                  tAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAatatat    >  1:1096465/1‑91 (MQ=255)                                                                                  tAACTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAatatat    <  1:477058/91‑1 (MQ=255)                                                                                    aCTTTCCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAg  >  1:1050525/1‑91 (MQ=255)                                                                                         |                                                                                      ATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAG  >  NZ_CP009273/3184373‑3184546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑