Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,965,761	A→G	D381G (GAC→GGC)	wecC →	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,965,761	0	A	G	100.0%	167.7 / NA	45	D381G (GAC→GGC)	wecC	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/30);  total (15/30)

GGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCA  >  NZ_CP009273/3965674‑3965848                                                                                        |                                                                                        ggCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGa                                                                                      <  1:844111/91‑1 (MQ=255) gccATAGCGGCGACACTCTGGTTGTTGAGCCTAACATCCGCCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGa                                                                                      <  2:67164/89‑1 (MQ=255)  gCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAg                                                                                     >  1:583153/1‑91 (MQ=255)    aTAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGgc                                                                                   >  2:245798/1‑91 (MQ=255)     tAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGgcg                                                                                  <  1:1039694/91‑1 (MQ=255)       gcggcgAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCt                                                                                >  1:717341/1‑91 (MQ=255)         ggcgAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTgg                                                                              >  2:243732/1‑91 (MQ=255)         ggcgAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTgg                                                                              >  2:487389/1‑91 (MQ=255)             aaaCTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAAc                                                                          <  2:717341/91‑1 (MQ=255)                        gttgAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGc                                                               <  2:370622/91‑1 (MQ=255)                                      ccaccaGTTGCCGAAAAAACTGTCCGGGCTTTGTACTCTGGCGCCGCTTGGCGAGGCGCGGGCAACGCCAGACGTGCTTGTTATGCTGGTc                                                 <  2:639036/91‑1 (MQ=255)                                      ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  1:566527/91‑1 (MQ=255)                                      ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  2:944053/91‑1 (MQ=255)                                      ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  2:95940/91‑1 (MQ=255)                                      ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  1:800547/91‑1 (MQ=255)                                      ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  2:98234/91‑1 (MQ=255)                                      ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  2:1121072/91‑1 (MQ=255)                                      ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  2:116837/91‑1 (MQ=255)                                      ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  2:151140/91‑1 (MQ=255)                                       caccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGGCATGCTGGTCg                                                >  2:487020/1‑91 (MQ=255)                                            gTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  1:99165/85‑1 (MQ=255)                                            gTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 >  2:99165/1‑85 (MQ=255)                                             ttGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGCTGCTGGTCGATCATa                                          >  2:184214/1‑91 (MQ=255)                                                ccGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTc                                       <  2:226178/91‑1 (MQ=255)                                                ccGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTc                                       <  1:1124100/91‑1 (MQ=255)                                                ccGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTc                                       <  1:362317/91‑1 (MQ=255)                                                       aaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 <  2:560427/74‑1 (MQ=255)                                                       aaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                 >  1:560427/1‑74 (MQ=255)                                                       aaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCaa                                <  2:605834/91‑1 (MQ=255)                                                            aCCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTa                           <  1:1008664/91‑1 (MQ=255)                                                                         ctctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACaa              <  1:1076977/91‑1 (MQ=255)                                                                          tctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAAt             <  2:999038/91‑1 (MQ=255)                                                                          tctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAAt             <  2:28143/91‑1 (MQ=255)                                                                          tctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAAt             >  1:460991/1‑91 (MQ=255)                                                                           ctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATg            >  2:588933/1‑91 (MQ=255)                                                                           ctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATg            >  1:398111/1‑91 (MQ=255)                                                                            tGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGt           <  2:562739/91‑1 (MQ=255)                                                                            tGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGt           <  2:385675/91‑1 (MQ=255)                                                                             ggCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTc                                       >  2:157385/1‑62 (MQ=255)                                                                             ggCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTc                                       <  1:157385/62‑1 (MQ=255)                                                                             ggCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTc          >  1:376352/1‑91 (MQ=255)                                                                              gcgcAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTcc         >  2:17773/1‑91 (MQ=255)                                                                              gcgcAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTcc         >  1:865867/1‑91 (MQ=255)                                                                                    cTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATcagc   <  2:376352/91‑1 (MQ=255)                                                                                     ttGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTAt                          <  1:240997/67‑1 (MQ=255)                                                                                     ttGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATcagca  <  2:238962/91‑1 (MQ=255)                                                                                        |                                                                                        GGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCA  >  NZ_CP009273/3965674‑3965848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑