Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,165,051	A→C	intergenic (‑97/‑265)	coaA ← / → BW25113_RS20630	type I pantothenate kinase/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,165,051	0	A	C	100.0%	105.8 / NA	29	intergenic (‑97/‑265)	coaA/BW25113_RS20630	type I pantothenate kinase/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/15);  total (14/15)

GGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAG  >  NZ_CP009273/4164970‑4165138                                                                                  |                                                                                        ggTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATggg                                                                                >  2:678124/1‑91 (MQ=255)            tATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAataata                                                                     >  1:1092419/1‑91 (MQ=255)             aTCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAAtaataa                                                                    >  1:277554/1‑91 (MQ=255)                 gCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGaaa                                                                <  1:18786/91‑1 (MQ=255)                      gCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAAtc                                                           <  1:221280/91‑1 (MQ=255)                       cGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAAtct                                                          >  2:188412/1‑91 (MQ=255)                        gATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAAtctc                                                         <  2:589566/91‑1 (MQ=255)                                       gcgTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATc                                          <  2:183746/91‑1 (MQ=255)                                              aGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGaaa                                   >  2:523533/1‑91 (MQ=255)                                               gTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGaaaa                                  <  1:462563/91‑1 (MQ=255)                                                 tCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGc                                <  1:678124/91‑1 (MQ=255)                                                  cTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCa                               >  1:923049/1‑91 (MQ=255)                                                   tGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCaa                              <  2:759511/91‑1 (MQ=255)                                                    gTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                             >  1:207749/1‑91 (MQ=255)                                                    gTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                             >  1:156693/1‑91 (MQ=255)                                                    gTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                             >  1:609160/1‑91 (MQ=255)                                                    gTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                             >  1:62563/1‑91 (MQ=255)                                                       gAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtgtg                          <  2:335142/91‑1 (MQ=255)                                                       gAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtgtg                          <  2:923049/91‑1 (MQ=255)                                                          tGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACa                       <  2:1092419/91‑1 (MQ=255)                                                               tAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGatat                  <  2:215776/91‑1 (MQ=255)                                                                     gCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc            >  2:614864/1‑91 (MQ=255)                                                                     gCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc            >  1:118570/1‑91 (MQ=255)                                                                      cAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCt           >  1:233666/1‑91 (MQ=255)                                                                       aggaggGTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTg          >  1:69176/1‑91 (MQ=255)                                                                             gTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaaaa    <  1:334375/91‑1 (MQ=255)                                                                             gTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaaaa    <  2:895753/91‑1 (MQ=255)                                                                             gTAACCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaaaa    <  2:903759/91‑1 (MQ=255)                                                                               aaCCCCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATGAAAAg  <  1:1032932/91‑1 (MQ=255)                                                                                  |                                                                                        GGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAG  >  NZ_CP009273/4164970‑4165138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑