Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	832,557	T→C	S251P (TCC→CCC)	hcxB →	hydroxycarboxylate dehydrogenase HcXB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	832,557	0	T	C	100.0%	123.9 / NA	35	S251P (TCC→CCC)	hcxB	hydroxycarboxylate dehydrogenase HcXB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/15);  total (20/15)

GTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACC  >  NZ_CP009273/832472‑832641                                                                                      |                                                                                     gTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCcggc                                                                                 <  2:1066181/91‑1 (MQ=255)   cGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCcggcgg                                                                               >  1:575978/1‑91 (MQ=255)                   gTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAg                                                               <  1:547082/91‑1 (MQ=255)                        ccTTTGCCGAACATAAAGTCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAAc                                                          <  1:225434/91‑1 (MQ=255)                        ccTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAAc                                                          <  2:947168/91‑1 (MQ=255)                        ccTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAAc                                                          <  2:575978/91‑1 (MQ=255)                        ccTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAAc                                                          <  2:547259/91‑1 (MQ=255)                           ttGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGtt                                                       >  1:1070810/1‑91 (MQ=255)                             gCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTAc                                                     <  2:711680/91‑1 (MQ=255)                                     tAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGCCGCATCAGGCAACGTTACAACCCCGt                                             >  2:681399/1‑91 (MQ=255)                                            tACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATg                                      >  1:870801/1‑91 (MQ=255)                                            tACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATg                                      >  1:594902/1‑91 (MQ=255)                                             aCGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCCTCAGGAAACGTTACAAACCAGTCCCGATGc                                     >  1:979300/1‑91 (MQ=255)                                             aCGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGc                                     >  2:1039331/1‑91 (MQ=255)                                             aCGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGc                                     <  1:395941/91‑1 (MQ=255)                                             aCGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGc                                     >  2:171626/1‑91 (MQ=255)                                             aCGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGc                                     <  1:169065/91‑1 (MQ=255)                                                   ttGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCt                               >  2:100802/1‑91 (MQ=255)                                                   ttGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCt                               >  1:733616/1‑91 (MQ=255)                                                   ttGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCt                               >  1:1122431/1‑91 (MQ=255)                                                   ttGCAGCGATGTGTGAAATTCTTGGCGGGGAGCTTCCCGGTGGCCAAACGACGCATCAGGCACCGTTTCAAACCAGGCCCGCTGCCATTCt                               >  2:522133/1‑91 (MQ=255)                                                        gCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACt                          <  1:1049768/91‑1 (MQ=255)                                                          gATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACTTTACAAACCAGTCCCGATGCCATTCTTACCTGc                        >  2:900407/1‑91 (MQ=255)                                                          gATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGc                        >  1:713893/1‑91 (MQ=255)                                                          gATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGc                        >  2:52754/1‑91 (MQ=255)                                                          gATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGc                        >  2:418254/1‑91 (MQ=255)                                                          gATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGc                        <  1:100802/91‑1 (MQ=255)                                                          gATGTGTGAAATTCTTGGCGGGGCGCTTCCCAGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGc                        >  2:1066736/1‑91 (MQ=255)                                                            tgtgtgAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAg                                              <  2:675722/67‑1 (MQ=255)                                                            tgtgtgAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAg                                              >  1:675722/1‑67 (MQ=255)                                                             gtgtgAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATg                     >  1:414582/1‑91 (MQ=255)                                                               gtgAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGc                                     >  2:405093/1‑73 (MQ=255)                                                               gtgAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGc                                     <  1:405093/73‑1 (MQ=255)                                                                  aaaTTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTACCTGCATGACCAc                <  1:1096299/91‑1 (MQ=255)                                                                            cGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatcatcatc      <  1:1039331/91‑1 (MQ=255)                                                                               ggCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAAc   >  1:334069/1‑91 (MQ=255)                                                                                gcgcTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAAcc  >  1:235857/1‑91 (MQ=255)                                                                                      |                                                                                     GTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACC  >  NZ_CP009273/832472‑832641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑