Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	871,743	A→G	E318G (GAA→GGA)	dgcI →	GGDEF domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	871,743	0	A	G	100.0%	100.2 / NA	28	E318G (GAA→GGA)	dgcI	GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/9);  total (19/9)

ATGTATAACTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGAAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTAACGACACCTTCGGTCATTTGCTGGGCGATGAAGTGT  >  NZ_CP009273/871661‑871829                                                                                   |                                                                                       atgtatAACTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCg                                                                                >  2:362079/1‑91 (MQ=255)    tataACTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGtt                                                                             >  1:224019/1‑91 (MQ=255)      taACTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTAt                                                                           >  1:586360/1‑91 (MQ=255)        aCTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTAttt                                                                         <  1:274880/91‑1 (MQ=255)        aCTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTAttt                                                                         >  1:717511/1‑91 (MQ=255)        aCTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTAttt                                                                         >  1:627834/1‑91 (MQ=255)         cTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTtg                                                                        >  1:1007784/1‑91 (MQ=255)         cTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTtg                                                                        >  2:580574/1‑91 (MQ=255)           cGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTtgtg                                                                      >  1:377448/1‑91 (MQ=255)           cGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTtgtg                                                                      >  1:713042/1‑91 (MQ=255)           cGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTtgtg                                                                      >  1:371160/1‑91 (MQ=255)           cGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTtgtg                                                                      >  2:767774/1‑91 (MQ=255)           cGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTtgtg                                                                      >  2:20226/1‑91 (MQ=255)             gCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGcc                                                                    >  2:287852/1‑91 (MQ=255)                ttCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGCCTGt                                                                 >  2:395376/1‑91 (MQ=255)                          acCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGa                                                       >  2:681370/1‑91 (MQ=255)                          acCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGa                                                       >  1:1067372/1‑91 (MQ=255)                            cTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATa                                                     <  2:503824/91‑1 (MQ=255)                             tGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATaa                                                    <  1:92332/91‑1 (MQ=255)                                      cAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCaaaaa                                           <  1:312483/91‑1 (MQ=255)                                           ggCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTa                                      <  2:422587/91‑1 (MQ=255)                                                      cTTTGACGAAGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTAACGACACCTTc                           <  2:100105/91‑1 (MQ=255)                                                              aaGATTTAAAAGCGCTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTAACGACACCTTCGGTCAttt                   >  2:881486/1‑91 (MQ=255)                                                                          cgcTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTAACGACACCTTCGGTCATTTGCTGGGCGATGa       <  1:654070/91‑1 (MQ=255)                                                                          cgcTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTAACGACACCTTCGGTCATTTGCTGGGCGATGa       >  2:699060/1‑91 (MQ=255)                                                                            cTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTAACGACACCTTCGGTCATTTGCTGGGCGATGAAg     >  2:220631/1‑91 (MQ=255)                                                                            cTGCCGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTAACGACACCTTCGGTCATTTGCTGGGCGATGAAg     <  2:742033/91‑1 (MQ=255)                                                                               ccGGGAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTAACGACACCTTCGGTCATTTGCTGGGCGATGAAgtgt  <  2:205667/91‑1 (MQ=255)                                                                                   |                                                                                       ATGTATAACTCGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGAAAAAGGCGGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAACATTAACGACACCTTCGGTCATTTGCTGGGCGATGAAGTGT  >  NZ_CP009273/871661‑871829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑