Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	928,200	T→C	R50R (CGT→CGC)	lrp →	leucine‑responsive transcriptional regulator Lrp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	928,200	0	T	C	100.0%	182.1 / NA	50	R50R (CGT→CGC)	lrp	leucine‑responsive transcriptional regulator Lrp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/30);  total (20/30)

GTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGTCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGT  >  NZ_CP009273/928116‑928283                                                                                     |                                                                                    gTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTg                                                                               >  2:551017/1‑91 (MQ=255) gTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTg                                                                               >  1:608765/1‑91 (MQ=255)  ttGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTgg                                                                              >  1:242528/1‑91 (MQ=255)   tGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGa                                                                             <  1:551017/91‑1 (MQ=255)   tGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGa                                                                             >  1:739629/1‑91 (MQ=255)          ggATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACaa                                                                      <  1:228694/91‑1 (MQ=255)          agATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACaa                                                                      <  2:757651/90‑1 (MQ=255)            aTGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAgg                                                                    >  1:1110589/1‑91 (MQ=255)            aTGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAgg                                                                    >  1:608513/1‑91 (MQ=255)                  gTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTAt                                                              >  1:749725/1‑91 (MQ=255)                    aattCTAACGTCGAGCTGTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                            <  2:357018/89‑1 (MQ=255)                    aTTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                            <  2:749725/91‑1 (MQ=255)                    aTTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                            <  2:608513/91‑1 (MQ=255)                    aTTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                            <  1:822489/91‑1 (MQ=255)                    aTTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                            <  1:1080345/91‑1 (MQ=255)                    aTTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                            <  1:680434/91‑1 (MQ=255)                     tttCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCa                                                           >  1:81494/1‑91 (MQ=255)                       tCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAgg                                                         <  1:647611/91‑1 (MQ=255)                         tAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGa                                                                             <  1:225898/69‑1 (MQ=255)                         tAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGa                                                                             >  2:225898/1‑69 (MQ=255)                         tAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACaa                                                                      >  2:1031968/1‑76 (MQ=255)                         tAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACaa                                                                      <  1:1031968/76‑1 (MQ=255)                              tCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATAc                                                  <  2:407906/91‑1 (MQ=255)                              tCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATAc                                                  <  1:249759/91‑1 (MQ=255)                               cGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                            <  2:447744/80‑1 (MQ=255)                               cGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                            >  1:447744/1‑80 (MQ=255)                                   cTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGgcgc                                             <  2:887999/91‑1 (MQ=255)                                       cTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCgctgct                                         <  2:619949/91‑1 (MQ=255)                                               gtgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAAccccc                                 >  2:784271/1‑91 (MQ=255)                                               gtgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAAccccc                                 >  1:982178/1‑91 (MQ=255)                                                tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCa                                <  1:967688/91‑1 (MQ=255)                                                 gggACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCAt                               <  1:784271/91‑1 (MQ=255)                                                      tttCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCt                          <  1:762644/91‑1 (MQ=255)                                                      tttCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCt                          >  1:436139/1‑91 (MQ=255)                                                       ttCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAGGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAAc                                     >  1:865510/1‑79 (MQ=255)                                                       ttCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAGGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAAc                                     <  2:865510/79‑1 (MQ=255)                                                               cGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATc                 <  1:831478/91‑1 (MQ=255)                                                               cGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATc                 <  2:368910/91‑1 (MQ=255)                                                               cGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATc                 <  1:763179/91‑1 (MQ=255)                                                               cGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATc                 <  1:965286/91‑1 (MQ=255)                                                                 ccGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCAc               >  2:84658/1‑91 (MQ=255)                                                                 ccGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCAc               >  2:808390/1‑91 (MQ=255)                                                                  cGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACt              <  2:1110589/91‑1 (MQ=255)                                                                   gTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGc                                                       >  1:690797/1‑49 (MQ=255)                                                                   gTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGc                                                       <  2:690797/49‑1 (MQ=255)                                                                   gTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACtt             >  1:561335/1‑91 (MQ=255)                                                                         tGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTa       <  2:886431/91‑1 (MQ=255)                                                                         tGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTa       <  1:101859/91‑1 (MQ=255)                                                                           aGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTAtt     <  1:307753/91‑1 (MQ=255)                                                                              gtgtGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGt  >  2:1023489/1‑91 (MQ=255)                                                                                     |                                                                                    GTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGTCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGT  >  NZ_CP009273/928116‑928283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑