| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 310 | (T)9→8 | intergenic (+55/‑27) | thrL → / → thrA | thr operon leader peptide/bifunctional aspartate kinase/homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 302 | 0 | T | . | 98.3% | 252.4 / ‑1.7 | 59 | intergenic (+47/‑35) | thrL/thrA | thr operon leader peptide/bifunctional aspartate kinase/homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (33/25); total (34/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
TTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGG > NZ_CP009273/170‑436 | ttACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttc < 1:205900/139‑2 (MQ=255) aCAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttttt < 1:663747/138‑1 (MQ=255) gagTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttttcgac < 1:817671/139‑5 (MQ=255) tACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCa > 2:776497/1‑138 (MQ=255) caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc < 2:267351/139‑1 (MQ=255) caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc < 1:547895/139‑1 (MQ=255) caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc < 1:553954/139‑1 (MQ=255) ccATGAAACGCATTAGCACCGCCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttt < 1:552005/121‑1 (MQ=255) ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc > 2:811613/1‑136 (MQ=255) ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc < 1:811613/136‑1 (MQ=255) ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAg > 1:764693/1‑139 (MQ=255) ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAg > 2:593460/1‑139 (MQ=255) ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAg > 1:8061/1‑139 (MQ=255) ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGAGCGGGCttttttt > 2:552005/1‑121 (MQ=255) aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTaac < 2:764693/139‑1 (MQ=255) aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTaacaa > 2:392396/1‑139 (MQ=255) aGCACCACCATTACCACCACCATCCCCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc < 2:304604/138‑1 (MQ=255) aGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc < 1:409876/138‑1 (MQ=255) ccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGtt > 2:489141/1‑138 (MQ=255) ccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGAAACGAGGTAACAACCATGCGAGTGtt > 1:863094/1‑138 (MQ=255) ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGtt < 2:8061/138‑1 (MQ=255) accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcgg > 1:106268/1‑139 (MQ=255) accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcgg > 1:747389/1‑139 (MQ=255) catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa > 1:696697/1‑139 (MQ=255) ttACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg > 2:25755/1‑139 (MQ=255) tACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACCTCAGTg > 1:317789/1‑138 (MQ=255) ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg > 1:643889/1‑138 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg > 1:861686/1‑137 (MQ=255) gCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt > 1:718756/1‑139 (MQ=255) cGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGtttt > 2:699939/1‑139 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc < 2:499092/75‑1 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc > 1:863480/1‑75 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc > 1:499092/1‑75 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc < 2:863480/75‑1 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc > 2:705814/1‑139 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc > 2:721263/1‑139 (MQ=255) cgcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTaac > 1:31531/1‑76 (MQ=255) cgcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTaac < 2:31531/76‑1 (MQ=255) cgcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGa > 2:109391/1‑124 (MQ=255) cgcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGa < 1:109391/124‑1 (MQ=255) cgcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgtgt > 2:680431/1‑139 (MQ=255) cgcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgtgt > 2:483937/1‑139 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGc < 2:385845/139‑1 (MQ=255) tACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGcc > 2:679091/1‑139 (MQ=255) cAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCg < 1:776497/138‑1 (MQ=255) gAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGatat > 2:2270/1‑139 (MQ=255) aaCACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGt < 2:146238/62‑1 (MQ=255) aaCACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGt > 1:146238/1‑62 (MQ=255) acaGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg > 1:586554/1‑139 (MQ=255) aaaaaaGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAg < 1:705814/139‑1 (MQ=255) aaaGCCCTCTCCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCaa < 2:606161/139‑1 (MQ=255) aGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGAAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATg < 2:863094/139‑1 (MQ=255) cGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcag < 2:521869/139‑1 (MQ=255) gCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg > 1:419886/1‑139 (MQ=255) cACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg < 1:839810/91‑1 (MQ=255) cACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg > 2:839810/1‑91 (MQ=255) tGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa > 2:505108/1‑115 (MQ=255) tGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa < 1:505108/115‑1 (MQ=255) tGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAg < 2:285893/118‑1 (MQ=255) tGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAg > 1:285893/1‑118 (MQ=255) tGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggca > 1:388373/1‑130 (MQ=255) tGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggca < 2:388373/130‑1 (MQ=255) cGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTgg < 2:58164/139‑1 (MQ=255) cGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTgg < 1:832732/139‑1 (MQ=255) | TTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGG > NZ_CP009273/170‑436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |