Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,718,241	Δ1 bp	coding (1864/2013 nt)	ydhK →	FUSC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,718,240	0	G	.	100.0%	100.9 / NA	24	coding (1863/2013 nt)	ydhK	FUSC family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/11);  total (13/11)

CTGAACTGTTCTCATGTTGTCTGGCAATTGCGCGACTGGGAATCGCGTTCCGATCCGTTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCA  >  NZ_CP009273/1718103‑1718371                                                                                                                                          |                                                                                                                                    ctgAACTGTTCTCATGTTGTCTGGCAATTGCGCGACTGGGAATCGCGTTCCGATCCGTTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTggcgc                                                                                                                                    >  2:484384/1‑138 (MQ=255)      cTGTTCTCATGTTGTCTGGCAATTGCGCGACTGGGAATCGCGTTCCGATCCGTTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCacac                                                                                                                              >  2:526270/1‑139 (MQ=255)                    tCTGGCAATTGCGCGACTGGGAATCGCGTTCCGATCCGTTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACa                                                                                                                 <  1:150435/138‑1 (MQ=255)                      tGGCAATTGCGCGACTGGGAATCGCGTTCCGATCCGTTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCg                                                                                                              >  1:694422/1‑139 (MQ=255)                              gcgcGACTGGGAATCGCGTTCCGATCCGTTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGc                                                                                                       >  2:104378/1‑138 (MQ=255)                                             gcgTTCCGATCCGTTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGt                                                                                        <  2:694422/138‑1 (MQ=255)                                                   cGATCCGTTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGtcatcatc                                                                                 <  1:526270/139‑1 (MQ=255)                                                                    tACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAg                                                                <  1:690111/139‑1 (MQ=255)                                                                    tACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAg                                                                >  1:731985/1‑139 (MQ=255)                                                                         gATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTggc                                                           <  1:484384/139‑1 (MQ=255)                                                                                   tttCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTggcggc                                                        >  1:338036/1‑132 (MQ=255)                                                                                   tttCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTggcggc                                                        <  2:338036/132‑1 (MQ=255)                                                                                   tttCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTc                                                 >  2:524731/1‑139 (MQ=255)                                                                                             gCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGTCGGCTg                                        >  1:830562/1‑138 (MQ=255)                                                                                             gCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTg                                        <  1:814150/138‑1 (MQ=255)                                                                                                gggAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACt                                    >  2:371043/1‑139 (MQ=255)                                                                                                   aGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCt                                 >  2:826892/1‑139 (MQ=255)                                                                                                     tgctgagtgagCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCg                               >  2:14423/4‑139 (MQ=255)                                                                                                       atgaGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTggcgg                                           >  2:453108/1‑125 (MQ=255)                                                                                                       atgaGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTggcgg                                           <  1:453108/125‑1 (MQ=255)                                                                                                               gCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAAc                     >  2:822795/1‑139 (MQ=255)                                                                                                                cGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACt                    >  1:326014/1‑139 (MQ=255)                                                                                                                    gCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGa                 <  1:826892/138‑1 (MQ=255)                                                                                                                                  tCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCa  <  1:371043/139‑1 (MQ=255)                                                                                                                                  tCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCa  <  1:104378/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    CTGAACTGTTCTCATGTTGTCTGGCAATTGCGCGACTGGGAATCGCGTTCCGATCCGTTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCA  >  NZ_CP009273/1718103‑1718371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑