Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,732,351	(A)7→6	coding (251/1026 nt)	purR →	HTH‑type transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,732,345	0	A	.	100.0%	195.3 / NA	44	coding (245/1026 nt)	purR	HTH‑type transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (18/26);  total (18/26)

CGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGA  >  NZ_CP009273/1732216‑1732474                                                                                                                                  |                                                                                                                                  cGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGaaaaaattgc                                                                                                                          >  1:183299/1‑135 (MQ=255)        aGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGa                                                                                                                  <  1:341866/139‑1 (MQ=255)        aGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAATCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGa                                                                                                                  <  2:709157/139‑1 (MQ=255)         gAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGaa                                                                                                                 <  1:586982/139‑1 (MQ=255)               cacTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTa                                                                                                           <  2:183299/139‑1 (MQ=255)               cacTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTa                                                                                                           <  1:289611/139‑1 (MQ=255)                    ctCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACAccc                                                                                                      >  2:865297/1‑139 (MQ=255)                    ctCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACAccc                                                                                                      <  1:187643/139‑1 (MQ=255)                    ctCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACAccc                                                                                                      >  1:103435/1‑139 (MQ=255)                       cccTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGa                                                                                                   >  1:737768/1‑139 (MQ=255)                         cTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGAtt                                                                                                 <  1:601301/139‑1 (MQ=255)                                ggTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                          <  2:103435/139‑1 (MQ=255)                                 gTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                          >  1:365356/1‑138 (MQ=255)                                 gTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                          <  2:365356/138‑1 (MQ=255)                                  tGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAAt                                                                                        <  1:468689/139‑1 (MQ=255)                                     cgcgTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCg                                                                                     <  1:385917/139‑1 (MQ=255)                                     cgcgTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCg                                                                                     <  1:657171/139‑1 (MQ=255)                                       cgTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTg                                                                                   >  1:796041/1‑139 (MQ=255)                                            ccTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGaa                                                                                                                 <  1:430280/104‑1 (MQ=255)                                            ccTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGaa                                                                                                                 >  2:430280/1‑104 (MQ=255)                                                      aaCCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTa                                                                                                           >  2:124496/1‑100 (MQ=255)                                                      aaCCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTa                                                                                                           <  1:124496/100‑1 (MQ=255)                                                      aaCCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTgaga                                                                     >  2:709993/1‑138 (MQ=255)                                                       aCCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGaaa                                                                   >  2:712582/1‑139 (MQ=255)                                                            acCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCg                                                              <  2:676441/139‑1 (MQ=255)                                                            acCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCg                                                              <  2:50731/139‑1 (MQ=255)                                                                      tCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTg                                                    >  1:443109/1‑139 (MQ=255)                                                                      tCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTg                                                    >  1:810969/1‑139 (MQ=255)                                                                            tGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCgat                                               >  2:110985/1‑138 (MQ=255)                                                                              cTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCgatga                                             <  1:709993/138‑1 (MQ=255)                                                                                ggCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATgg                                          >  1:188635/1‑139 (MQ=255)                                                                                    aCCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGgcgc                                       >  1:816699/1‑138 (MQ=255)                                                                                           gcGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAAcgc                               >  1:603638/1‑139 (MQ=255)                                                                                                   gCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATgg                       >  2:527823/1‑139 (MQ=255)                                                                                                   gCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATgg                       >  1:292615/1‑139 (MQ=255)                                                                                                     cTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTc                     <  1:287235/139‑1 (MQ=255)                                                                                                           tGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTgg               >  1:526719/1‑139 (MQ=255)                                                                                                               gagaTCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGAt           <  1:865297/139‑1 (MQ=255)                                                                                                               gagaTCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGAt           <  1:527823/139‑1 (MQ=255)                                                                                                               gagaTCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGAt           <  1:457359/139‑1 (MQ=255)                                                                                                                   tCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATgtgt       <  2:810969/139‑1 (MQ=255)                                                                                                                   tCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATgtgt       <  2:816699/139‑1 (MQ=255)                                                                                                                        gAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTg   <  1:853708/138‑1 (MQ=255)                                                                                                                         aaGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGa  <  2:632601/138‑1 (MQ=255)                                                                                                                         aaGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGa  <  1:110985/138‑1 (MQ=255)                                                                                                                                  |                                                                                                                                  CGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGA  >  NZ_CP009273/1732216‑1732474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑