| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,438,552 | Δ1 bp | coding (488/810 nt) | yfcA ← | sulfite exporter TauE/SafE family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,438,551 | 0 | G | . | 81.6% | 105.6 / 27.1 | 38 | coding (489/810 nt) | yfcA | sulfite exporter TauE/SafE family protein |
| Reads supporting (aligned to +/- strand): ref base G (3/4); new base . (16/15); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01 | |||||||||||
TAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACA > NZ_CP009273/2438446‑2438685 | tAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGa > 1:217769/1‑139 (MQ=255) ccgATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGc > 2:617454/1‑138 (MQ=255) ccgATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGc > 1:209215/1‑138 (MQ=255) cgttgAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAAcgc < 2:82693/139‑1 (MQ=255) gttgAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGa < 1:192948/139‑1 (MQ=255) tAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTa < 1:364322/139‑1 (MQ=255) tAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTa < 2:542366/139‑1 (MQ=255) ggCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCg > 2:363772/1‑138 (MQ=255) tGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCgcag < 2:801922/69‑4 (MQ=255) tGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCgcag > 1:801922/1‑66 (MQ=255) aGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATc > 2:158406/1‑139 (MQ=255) tGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATa > 1:206750/1‑84 (MQ=255) tGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATa < 2:206750/84‑1 (MQ=255) tGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGgcgc > 2:780464/1‑139 (MQ=255) tGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGgcgc > 1:375576/1‑139 (MQ=255) cTTTGGCGAGGTTAAAGCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTgg < 2:499305/139‑1 (MQ=255) aGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGtcttc > 2:689143/1‑139 (MQ=255) aTCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCAcc < 2:82936/139‑1 (MQ=255) aTCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCAcc > 2:743982/1‑138 (MQ=255) aTCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCt < 1:795089/139‑1 (MQ=255) cacaGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCATAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGa > 1:629221/1‑93 (MQ=255) cacaGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGa < 2:629221/93‑1 (MQ=255) cacaGCGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCtt > 1:84620/1‑139 (MQ=255) cGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCgg > 2:273422/1‑108 (MQ=255) cGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCgg < 1:273422/108‑1 (MQ=255) cGTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCa > 1:350759/1‑139 (MQ=255) gTAACGAAGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCAt < 1:689143/139‑1 (MQ=255) aaGGCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCaa < 2:217769/139‑1 (MQ=255) aGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGc > 1:672258/1‑137 (MQ=255) gCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATc < 1:581215/132‑1 (MQ=255) gCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATc > 2:581215/1‑132 (MQ=255) gCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCAt < 2:264664/130‑1 (MQ=255) gCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCAt > 1:264664/1‑130 (MQ=255) gCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGa > 1:175003/1‑138 (MQ=255) gCCAGCGCGTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGa > 1:225953/1‑138 (MQ=255) gTAAAACGACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGc < 2:225953/139‑1 (MQ=255) aaCGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGa < 1:158406/139‑1 (MQ=255) gACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACa < 1:780464/139‑1 (MQ=255) gACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACa < 2:209215/139‑1 (MQ=255) gACCC‑GCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACa < 2:350759/139‑1 (MQ=255) | TAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACA > NZ_CP009273/2438446‑2438685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |