| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,771,232 | (A)6→5 | intergenic (+91/+273) | ypjF → / ← ypjA | type IV toxin‑antitoxin system toxin YpjF/adhesin‑like autotransporter YpjA/EhaD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,771,227 | 0 | A | . | 81.2% | 118.6 / 37.8 | 48 | intergenic (+86/+278) | ypjF/ypjA | type IV toxin‑antitoxin system toxin YpjF/adhesin‑like autotransporter YpjA/EhaD |
| Reads supporting (aligned to +/- strand): ref base A (4/5); new base . (17/22); total (21/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGG > NZ_CP009273/2771093‑2771359 | tCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGaaaa > 1:847304/1‑138 (MQ=255) tGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGaaaaacc > 2:268771/1‑137 (MQ=255) ggCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACAttt < 2:442239/139‑1 (MQ=255) ggCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACAtttt > 1:36839/1‑139 (MQ=255) ccGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCtttt < 2:847304/136‑1 (MQ=255) ggCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGAcc < 1:736882/139‑1 (MQ=255) ggCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGAcc < 2:679089/139‑1 (MQ=255) tGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACcaac > 1:577767/1‑138 (MQ=255) tAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTg > 1:158281/1‑137 (MQ=255) aaaTACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGa < 2:390740/137‑1 (MQ=255) tACAGAGCAGACTGAAGGAAATCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGAt < 2:673131/139‑1 (MQ=255) agCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTAcaa > 1:280739/1‑139 (MQ=255) agCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGaa > 1:11444/1‑125 (MQ=255) agCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGaa < 2:11444/125‑1 (MQ=255) agCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTAcaat > 1:450839/1‑139 (MQ=255) gCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTAcaatc < 2:577767/139‑1 (MQ=255) cAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAAtt < 1:149092/120‑1 (MQ=255) cAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAAtt > 2:149092/1‑120 (MQ=255) cAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCACCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTAcaatc > 1:771482/1‑138 (MQ=255) aGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGAt < 1:567814/124‑1 (MQ=255) aGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGAt > 2:567814/1‑124 (MQ=255) aGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTaaca < 2:46281/139‑1 (MQ=255) aGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTaaca < 1:495809/139‑1 (MQ=255) aaaGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAacaaca < 1:466237/139‑1 (MQ=255) aTAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAAtt > 1:145595/1‑136 (MQ=255) tCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGcc > 1:860490/1‑138 (MQ=255) tCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCcca > 1:15834/1‑139 (MQ=255) tCCCGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCcca > 1:513687/1‑139 (MQ=255) ccGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCccacc < 1:268771/139‑1 (MQ=255) ccGCTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCccac < 2:145595/138‑1 (MQ=255) cTGTAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAAt < 1:100813/139‑1 (MQ=255) tAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCccac > 2:861911/1‑132 (MQ=255) tAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCccac < 1:861911/132‑1 (MQ=255) tAACCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCAt > 1:193154/1‑139 (MQ=255) aCCTCCCCTCCCCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGa > 2:462927/1‑139 (MQ=255) ctcccctcccCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACaa < 1:622691/104‑1 (MQ=255) ctcccctcccCCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACaa > 2:622691/1‑104 (MQ=255) ccctccccCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGa < 2:193154/139‑1 (MQ=255) ccctccccCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGa < 1:143707/139‑1 (MQ=255) cctccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGa > 2:108966/1‑139 (MQ=255) cctccccCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGAc < 2:450839/139‑1 (MQ=255) cctccccCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGAc < 1:462927/139‑1 (MQ=255) cctccccCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGAc < 2:491168/139‑1 (MQ=255) cctccccCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGAc < 2:493331/139‑1 (MQ=255) cctccccCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGAc > 2:852259/1‑139 (MQ=255) cctccccCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGAc < 2:859095/139‑1 (MQ=255) ctccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGAc < 1:490566/139‑1 (MQ=255) tccccCG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACgg > 1:804627/1‑139 (MQ=255) ccccG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAAtt < 2:304622/56‑1 (MQ=255) ccccG‑AAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAAtt > 1:304622/1‑56 (MQ=255) | TCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGG > NZ_CP009273/2771093‑2771359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |