| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,987,561 | (A)7→6 | coding (264/477 nt) | BW25113_RS14845 → | lytic transglycosylase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,987,555 | 0 | A | . | 82.3% | 40.1 / 9.0 | 17 | coding (258/477 nt) | BW25113_RS14845 | lytic transglycosylase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base . (10/4); total (13/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
AGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAA > NZ_CP009273/2987448‑2987689 | aGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAAc > 2:103801/1‑135 (MQ=255) agaTAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAg > 2:850407/1‑139 (MQ=255) aTAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAgg > 1:148015/1‑138 (MQ=255) aCAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGAtt < 2:148015/139‑1 (MQ=255) aCAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGAt < 1:103801/138‑1 (MQ=255) taAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTtata > 1:313841/1‑138 (MQ=255) gTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCAt > 2:215463/1‑139 (MQ=255) ttAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCAtt > 2:660524/1‑139 (MQ=255) attattGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCa > 2:41893/1‑139 (MQ=255) attGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACg < 2:404701/138‑1 (MQ=255) tGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGt > 1:508349/1‑138 (MQ=255) tGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGt > 1:686468/1‑138 (MQ=255) tGCAGATAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGt > 2:501259/1‑138 (MQ=255) aTAAACCAGATGCATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTg > 1:164777/1‑139 (MQ=255) gATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGc > 1:309269/1‑139 (MQ=255) cATATTCCCCTGTT‑AAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTggg < 2:313841/139‑1 (MQ=255) tGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATaa > 1:284578/1‑139 (MQ=255) | AGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAA > NZ_CP009273/2987448‑2987689 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |