Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,030,529	Δ1 bp	noncoding (29/77 nt)	BW25113_RS19985 →	tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,030,529	0	A	.	100.0%	19.6 / NA	10	noncoding (29/77 nt)	BW25113_RS19985	tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (6/4);  total (6/4)

GTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTG  >  NZ_CP009273/4030395‑4030583                                                                                                                                       |                                                       gTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCcccct                                                    >  2:413786/1‑138 (MQ=21) gTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCcccct                                                    >  2:641590/1‑138 (MQ=21)    aCCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCCTGAt                                                <  1:398556/139‑1 (MQ=21)    aCCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCCTGAt                                                <  2:320388/139‑1 (MQ=21)                ccTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCCTGATAAGGGTGAGGTc                                    >  1:183872/1‑139 (MQ=21)                ccTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCCTGATAAGGGTGAGGTc                                    >  2:309205/1‑139 (MQ=21)                ccTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCCTGATAAGGGTGAGGTc                                    >  2:51916/1‑139 (MQ=21)                    cGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTGTGAAGTGCGCACACAGATTGTCTGATGAAAATGAGCAGTAAAGCCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCGTGATAAGGGTGAGGTCgg                                  <  2:679247/137‑1 (MQ=11)                                      accttaAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAgg               <  1:309205/138‑1 (MQ=21)                                          taAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTAc          >  2:274474/1‑139 (MQ=21)                                          taAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTAc          >  2:611721/1‑139 (MQ=21)                                                  cGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGC‑CCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTg  >  1:531037/1‑139 (MQ=21)                                                                                                                                       |                                                       GTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTG  >  NZ_CP009273/4030395‑4030583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑