| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,262,978 | (C)5→4 | coding (822/2823 nt) | uvrA ← | excinuclease ABC subunit UvrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,262,974 | 0 | C | . | 97.6% | 174.8 / ‑4.3 | 42 | coding (826/2823 nt) | uvrA | excinuclease ABC subunit UvrA |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (21/20); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCGGGTCGTCCATATCCGCCACTACCG > NZ_CP009273/4262853‑4263105 | taGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTaaa > 2:232465/1‑139 (MQ=255) aaGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGa < 1:804842/139‑1 (MQ=255) gcggcgATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGaaaa > 2:321338/1‑137 (MQ=255) aCGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGtt < 2:847242/139‑1 (MQ=255) gATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCAc > 2:731073/1‑139 (MQ=255) aTCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCGGTCGCAGGTCGGGGAGG‑CCGCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg < 2:357621/139‑1 (MQ=255) aTCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg < 2:773227/139‑1 (MQ=255) gCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCa < 1:540817/138‑1 (MQ=255) tccaccaGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGTTTCGAAACAGTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCa < 2:54009/135‑1 (MQ=255) gccagcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTGGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGt < 2:50366/110‑1 (MQ=255) gccagcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGt > 1:50366/1‑110 (MQ=255) gccagcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGcc > 2:56032/1‑139 (MQ=255) gTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTggg < 1:747531/139‑1 (MQ=255) ttCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGtt > 2:504743/1‑112 (MQ=255) ttCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGtt < 1:504743/112‑1 (MQ=255) ttCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCAAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGaa < 1:1852/139‑1 (MQ=255) tGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTcc < 2:249935/98‑1 (MQ=255) tGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTcc > 1:249935/1‑98 (MQ=255) tGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGtt > 2:657332/1‑139 (MQ=255) gATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTgg > 1:118001/1‑139 (MQ=255) tCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCg < 2:151480/139‑1 (MQ=255) cTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCt < 2:298620/89‑1 (MQ=255) cTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCt > 1:298620/1‑89 (MQ=255) cTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGgag < 1:710882/139‑1 (MQ=255) gATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg > 2:763237/1‑96 (MQ=255) gATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg < 1:763237/96‑1 (MQ=255) gttCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACagcagc > 1:262823/3‑139 (MQ=255) ttGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg > 1:607703/1‑80 (MQ=255) ttGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg < 2:607703/80‑1 (MQ=255) ttGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCtt > 2:680449/1‑139 (MQ=255) ttGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCtt > 2:603125/1‑139 (MQ=255) tACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg < 2:251410/74‑1 (MQ=255) tACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg > 1:251410/1‑74 (MQ=255) tACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCggg < 1:321338/138‑1 (MQ=255) tACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCggg < 1:232465/138‑1 (MQ=255) tACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCGGgt > 2:53348/1‑139 (MQ=255) tACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCGGgt > 2:436927/1‑139 (MQ=255) cGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCGGgtcg > 2:109051/1‑139 (MQ=255) aGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTACGCTTTCGGGGCGTCCa > 2:284439/1‑138 (MQ=255) ggCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCGGGTCGTCCata > 1:859660/1‑139 (MQ=255) tCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCGGGTCGTCCATATCCGc < 1:657332/139‑1 (MQ=255) cGGGCA‑GCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATGGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCGGGTCGTCCATATCCGCCACTACcg < 2:257615/139‑1 (MQ=255) | TAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGCAGCTCTTCCGCTTTCGGGTCGTCCATATCCGCCACTACCG > NZ_CP009273/4262853‑4263105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |