Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,292,953	+C	coding (322/444 nt)	yhbP ←	YhbP family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,292,952	1	.	C	97.8%	158.3 / ‑3.9	46	coding (323/444 nt)	yhbP	YhbP family protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (26/19);  total (27/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑G‑CAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGG  >  NZ_CP009273/3292816‑3293084                                                                                                                                             |                                                                                                                                     tttAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑G‑cc                                                                                                                                    <  2:799116/139‑2 (MQ=255) tttAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑G‑c                                                                                                                                     >  2:555440/1‑138 (MQ=255)         cgAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGa                                                                                                                              >  2:701731/1‑138 (MQ=255)           aGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAg                                                                                                                           >  1:628609/1‑139 (MQ=255)           aGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACC‑AAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGc                                                                                                                          <  1:854715/139‑1 (MQ=255)              cGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGcga                                                                                                                        <  1:362710/139‑1 (MQ=255)              cGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGcga                                                                                                                        <  2:406946/139‑1 (MQ=255)               gTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGcgac                                                                                                                       <  1:555440/139‑1 (MQ=255)                        gCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACg                                                                                                              >  2:43585/1‑139 (MQ=255)                        gCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACg                                                                                                              >  2:514548/1‑139 (MQ=255)                        gCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACg                                                                                                              >  2:43558/1‑139 (MQ=255)                                ccTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTgcgc                                                                                                      >  1:181405/1‑139 (MQ=255)                                   gAGTCGCGTAGCCAAATCATTTTTTTTACCAAACCCAAGCGTGTTGCCGTTAATTTTGATTT‑ATCGGAGCGGATTTTCCAAACCCGGGCCCACCAGCATTCTGCC‑CAACCGGAATGCAAGAATTGTACGCCTTccgcgc                                                                                                    >  2:544808/1‑139 (MQ=255)                                        gcgTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                                              <  1:234287/139‑1 (MQ=255)                                        gcgTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                                              >  2:188809/1‑139 (MQ=255)                                        gcgTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                                              >  1:729278/1‑139 (MQ=255)                                        gcgTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                                              >  1:600218/1‑139 (MQ=255)                                        gcgTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                                              >  2:827796/1‑139 (MQ=255)                                         cgTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCg                                                                                             <  1:626603/139‑1 (MQ=255)                                          gTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGc                                                                                            >  2:724807/1‑139 (MQ=255)                                                  aTCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCa                                                                                    >  1:862136/1‑139 (MQ=255)                                                         tttttACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTc                                                                               <  2:247661/138‑1 (MQ=255)                                                              aCCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCt                                                                         >  1:116570/1‑139 (MQ=255)                                                                      ccAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtct                                                                 <  1:514548/139‑1 (MQ=255)                                                                      ccAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtct                                                                 <  1:724807/139‑1 (MQ=255)                                                                      ccAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtct                                                                 <  1:43585/139‑1 (MQ=255)                                                                               tgtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTa                                                         >  2:64148/1‑138 (MQ=255)                                                                                   ggTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGcga                                                                                                                        >  1:446982/1‑71 (MQ=255)                                                                                   ggTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGcga                                                                                                                        <  2:446982/71‑1 (MQ=255)                                                                                     tGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGca                                                  <  1:648826/139‑1 (MQ=255)                                                                                     tGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGca                                                  <  1:488201/139‑1 (MQ=255)                                                                                         tttGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACAcc                                              <  2:94963/139‑1 (MQ=255)                                                                                             aTTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcgcg                                           >  1:788305/1‑138 (MQ=255)                                                                                                                  caccCCGGGGCCGC‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCTCCTTCCATCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGc                     <  1:511553/139‑1 (MQ=255)                                                                                                                  cacaCCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGc                     <  1:731539/139‑1 (MQ=255)                                                                                                                     acCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGa                  <  1:701731/139‑1 (MQ=255)                                                                                                                     acCGGGGCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGa                  <  1:298226/139‑1 (MQ=255)                                                                                                                        ggggCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtct                                                                 >  1:851876/1‑89 (MQ=255)                                                                                                                        ggggCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtct                                                                 <  2:851876/89‑1 (MQ=255)                                                                                                                         gggCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt              >  1:688382/1‑139 (MQ=255)                                                                                                                         gggCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt              >  1:17363/1‑139 (MQ=255)                                                                                                                         gggCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt              >  1:167419/1‑139 (MQ=255)                                                                                                                          ggCCGA‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt             >  1:264488/1‑139 (MQ=255)                                                                                                                               a‑CAGCATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCg        >  2:218665/1‑139 (MQ=255)                                                                                                                                    cATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTcc    >  2:862979/1‑139 (MQ=255)                                                                                                                                    cATTCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTcc    >  1:156396/1‑139 (MQ=255)                                                                                                                                      ttCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCgg  >  1:89482/1‑139 (MQ=255)                                                                                                                                      ttCTG‑GCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAACCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCgg  >  1:343722/1‑139 (MQ=255)                                                                                                                                             |                                                                                                                                     TTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGA‑CAGCATTCTG‑G‑CAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGG  >  NZ_CP009273/3292816‑3293084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑