Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927330,5310AC69.0% 98.4 / 53.4 58N294T (AAC→ACC) carAglutamine‑hydrolyzing carbamoyl‑phosphate synthase small subunit
Reads supporting (aligned to +/- strand):  ref base A (6/12);  new base C (18/22);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGA  >  NZ_CP009273/30396‑30651
                                                                                                                                       |                                                                                                                        
aTTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCa                                                                                                                       <  2:669537/139‑1 (MQ=255)
 ttACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCAt                                                                                                                      <  2:350116/139‑1 (MQ=255)
 ttACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCAt                                                                                                                      <  1:529476/139‑1 (MQ=255)
    cGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCg                                                                                                                   >  2:318394/1‑139 (MQ=255)
        aTTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTa                                                                                                               <  1:413433/139‑1 (MQ=255)
            ccGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAg                                                                                                            <  2:727295/138‑1 (MQ=255)
                   ccAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGAtgtg                                                                                                       >  2:83989/1‑136 (MQ=255)
                     aGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGaa                                                                                                  <  1:557546/139‑1 (MQ=255)
                        aaTTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGaaaaa                                                                                               <  1:83989/139‑1 (MQ=255)
                          ttCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACg                                                                                             <  2:194868/139‑1 (MQ=255)
                            ccTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCAt                                                                                                                      >  1:848265/1‑112 (MQ=255)
                            ccTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCAt                                                                                                                      <  2:848265/112‑1 (MQ=255)
                            ccTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATc                                                                                                                     >  2:539812/1‑113 (MQ=255)
                            ccTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATc                                                                                                                     <  1:539812/113‑1 (MQ=255)
                             cTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTg                                                                                           <  1:56824/138‑1 (MQ=255)
                               cGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGt                                                                                         >  1:675843/1‑138 (MQ=255)
                                      gATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCa                                                                                 <  1:367744/139‑1 (MQ=255)
                                      gATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCa                                                                                 <  2:67749/139‑1 (MQ=255)
                                      gATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCa                                                                                 <  1:384487/139‑1 (MQ=255)
                                       atatTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCAc                                                                                >  1:464868/1‑139 (MQ=255)
                                               gTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCAt                                                                                                                      <  2:56188/93‑1 (MQ=255)
                                               gTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCAt                                                                                                                      >  1:56188/1‑93 (MQ=255)
                                               gTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAg                                                                         >  1:849861/1‑138 (MQ=255)
                                                  ttCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAAc                                                                      >  2:498680/1‑138 (MQ=255)
                                                          cTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGtttt                                                             >  2:579071/1‑139 (MQ=255)
                                                            gTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGc                                                           >  1:571665/1‑139 (MQ=255)
                                                            gTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCAt                                                                                                                      >  1:371123/1‑80 (MQ=255)
                                                            gTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCAt                                                                                                                      <  2:371123/80‑1 (MQ=255)
                                                                     atcaGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACg                                                   <  1:453960/138‑1 (MQ=255)
                                                                     atcaGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGa                                                  <  1:846310/139‑1 (MQ=255)
                                                                      tcaGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGaa                                                 <  2:849861/139‑1 (MQ=255)
                                                                       caGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAg                                                >  1:156992/1‑139 (MQ=255)
                                                                       caGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAg                                                >  2:222712/1‑139 (MQ=255)
                                                                       caGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAg                                                >  1:259714/1‑139 (MQ=255)
                                                                                gcgctggcgAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACAtt                                         <  2:804741/137‑1 (MQ=255)
                                                                                      gcgAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCa                                  <  1:222712/138‑1 (MQ=255)
                                                                                           cGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGAtgt                                                                                                        >  1:463134/1‑63 (MQ=255)
                                                                                           cGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGAtgt                                                                                                        <  2:463134/63‑1 (MQ=255)
                                                                                           cGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTg                            <  1:318394/139‑1 (MQ=255)
                                                                                                cggagtcTGTCAAAATGGTATTTGGACACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCgtg                        <  1:354545/132‑1 (MQ=255)
                                                                                                cGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCgtgt                       <  1:750458/139‑1 (MQ=255)
                                                                                                cGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCgtgt                       >  1:117822/1‑139 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGaa                                                                                                  >  2:518710/1‑56 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGaa                                                                                                  <  1:518710/56‑1 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCa                                                                                 <  1:36126/73‑1 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCa                                                                                 >  2:36126/1‑73 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGGGGTGGACGAAGCAACATTAc                                       <  2:808772/115‑1 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGGGGTGGACGAAGCAACATTAc                                       >  1:808772/1‑115 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGa                                                     <  1:369587/101‑1 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGa                                                     >  2:369587/1‑101 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCAt                >  2:162631/1‑138 (MQ=255)
                                                                                                        gTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCAt                >  1:256808/1‑138 (MQ=255)
                                                                                                         tCAAAATGAAATTTGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATa               <  2:337070/138‑1 (MQ=255)
                                                                                                                 aaaTTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGa                                                     >  2:16456/1‑92 (MQ=255)
                                                                                                                 aaaTTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGa                                                     <  1:16456/92‑1 (MQ=255)
                                                                                                                      tGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa  <  1:468672/138‑1 (MQ=255)
                                                                                                                      tGGTCACCACGGCGGCACCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa  <  2:587955/138‑1 (MQ=255)
                                                                                                                      tGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa  <  1:214093/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                        
ATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGA  >  NZ_CP009273/30396‑30651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑