Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927397,9860TC72.5% 70.4 / 43.0 51R245R (CGT→CGC) murCUDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (9/5);  new base C (18/19);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.18e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCCAGG  >  NZ_CP009273/97857‑98120
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ctaccAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCa                                                                                                                                 >  2:776621/1‑137 (MQ=255)
ctaccAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCa                                                                                                                                 <  1:776621/137‑1 (MQ=255)
   ccAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACt                                                                                                                            >  2:627287/1‑139 (MQ=255)
      gggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCa                                                                                                                                 <  2:839297/131‑1 (MQ=255)
      gggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCa                                                                                                                                 >  1:839297/1‑131 (MQ=255)
      gggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCa                                                                                                                                 >  1:264415/1‑131 (MQ=255)
      gggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCa                                                                                                                                 <  2:264415/131‑1 (MQ=255)
          gACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGAc                                                                                                                                   >  1:298203/1‑125 (MQ=255)
          gACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGAc                                                                                                                                   <  2:298203/125‑1 (MQ=255)
           aCTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCtt                                                                                                                    >  2:742958/1‑139 (MQ=255)
            cTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCtt                                                                                                                    >  2:92983/1‑138 (MQ=255)
            cTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCtt                                                                                                                    >  2:842674/1‑138 (MQ=255)
             tttGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCtt                                                                                                                    >  1:117920/1‑137 (MQ=255)
                 agaATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGa                                                                                                              <  1:70380/139‑1 (MQ=255)
                   aaTTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAg                                                                                                            <  1:543297/139‑1 (MQ=255)
                   aaTTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAg                                                                                                            <  2:282042/139‑1 (MQ=255)
                           aCAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAc                                                                                                    <  2:692377/139‑1 (MQ=255)
                           aCAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAc                                                                                                    <  1:128605/139‑1 (MQ=255)
                           aCAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAc                                                                                                    <  1:863992/139‑1 (MQ=255)
                            cAGTCTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAcg                                                                                                   <  1:122918/139‑1 (MQ=255)
                             aGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAcgt                                                                                                  <  2:374460/139‑1 (MQ=255)
                               aCTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgc                                                                                                >  1:352627/1‑139 (MQ=255)
                                 ttttATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgcgt                                                                                              >  2:381271/1‑139 (MQ=255)
                                             tCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTAt                                                                                  >  1:668344/1‑139 (MQ=255)
                                                          ccGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGcc                                                                     <  1:627287/139‑1 (MQ=255)
                                                                tACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAg                                                                <  1:92983/138‑1 (MQ=255)
                                                                  cGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAggg                                                              <  2:120856/138‑1 (MQ=255)
                                                                        tGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGCGTGGTGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACtt                                                        >  2:632021/1‑138 (MQ=255)
                                                                        tGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACtt                                                        >  2:451248/1‑138 (MQ=255)
                                                                               aTGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgc                                                >  1:620118/1‑139 (MQ=255)
                                                                               aTGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgc                                                >  1:114542/1‑139 (MQ=255)
                                                                               aTGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgc                                                <  2:196881/139‑1 (MQ=255)
                                                                                 gtgtgtTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGctgctg                                              >  2:818256/1‑139 (MQ=255)
                                                                                  tgtgtTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATcag                                                                               >  2:196840/1‑105 (MQ=255)
                                                                                  tgtgtTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATcag                                                                               <  1:196840/105‑1 (MQ=255)
                                                                                  tgtgtTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCtgctgc                                             >  1:465366/1‑139 (MQ=255)
                                                                                  tgtgtTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAcgt                                                                                                  >  2:467094/1‑86 (MQ=255)
                                                                                  tgtgtTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGAcgt                                                                                                  <  1:467094/86‑1 (MQ=255)
                                                                                  tgtgtTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCtgctgc                                             >  2:420068/1‑139 (MQ=255)
                                                                                    tgtTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTgcgc                                           >  2:566661/1‑139 (MQ=255)
                                                                                            atCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCa                                                                                                                                 <  2:272945/45‑1 (MQ=255)
                                                                                            atCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCa                                                                                                                                 >  1:272945/1‑45 (MQ=255)
                                                                                              ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa                                    >  1:590421/1‑136 (MQ=255)
                                                                                                ggTGATCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAagag                               >  1:662535/1‑139 (MQ=255)
                                                                                                     tCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGAt                          <  2:620118/139‑1 (MQ=255)
                                                                                                     tCCGCGAATTGTTACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGAt                          <  1:451248/139‑1 (MQ=255)
                                                                                                                ttACCGCGAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCAcc                <  1:818256/138‑1 (MQ=255)
                                                                                                                 tagcgcgAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCt              >  1:446399/4‑139 (MQ=255)
                                                                                                                     gcgAGTGGGGCGCCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAAt          <  2:114542/139‑1 (MQ=255)
                                                                                                                             ggCGCCAGACCACGGCTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCCAgg  <  1:720578/139‑1 (MQ=255)
                                                                                                                             ggCGCCAGACCACGACTTCCGGCTTCAGCGAAGATGCCGACGGGCGTGTAGAAGATTATCAGCGGATTGGCCCGCAGGGGCCCTTTACGCTGCTGGGCCAGGACAAAGAGCGGATGGGCGCCACCCTGAATGCCCCAgg  >  2:720578/1‑139 (MQ=255)
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CTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCCAGG  >  NZ_CP009273/97857‑98120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑