| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,601 | 0 | C | A | 57.1% | ‑0.6 / 22.8 | 21 | L267F (TTG→TTT) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base C (8/1); new base A (0/12); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACC‑GTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTC > NZ_CP009273/353464‑353717 | cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACC‑GTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGc‑aac > 2:600053/1‑138 (MQ=255) cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACC‑GTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAAcc > 1:539821/1‑139 (MQ=255) ttCCAGCCGCAGACCTGTGTCAGCGGCTACC‑GTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTtc > 1:123775/1‑139 (MQ=255) ccACCCGCAGCCCTGTGTCAGCGGCTCCC‑GTGATTCTTTCCCGCCAACACCCGCGCATTTACCCAACGCCATGTGAAAAAATGCCTCCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGC‑AAAAAAACCGCCGTtctc < 1:600053/139‑1 (MQ=255) ccGCAGACCTGTGTCAGCGGCTACC‑GTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTtctctc > 1:319008/1‑137 (MQ=255) ccggtgtGAGC‑GCTACCGGTAATTCATTCCCCCCACCACCCGCCATTTTACCAAACCCCATGTGAAAAAATCCCTCCGGGGGGGTTGTCGGCAGGTTTTTTTCCCGCCGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTgg < 2:847416/136‑1 (MQ=255) gCGGCTACC‑GTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAag > 2:157204/1‑139 (MQ=255) gCGGCTACC‑GTGATTCATTTCCGCCAACAACCGAGCATTTATCAAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCgggagac > 1:136667/1‑134 (MQ=255) tACC‑GTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGA‑Gaa > 1:240432/1‑138 (MQ=255) ccc‑GTGATTTATTCCCCCCAACACCCGCGCTTTTCCCCAACGCCAGGTGCAAAAAGCCCTCCGCGGCGGTTGTCGGCCGTCTTTTTGTCCGCCAA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAAt < 2:418636/138‑1 (MQ=255) aTTCCCCCCAACACCCGCGCATTTCTCCACCGCCATGTGAAAAAATGCCTCCGCGGCGCCTGCCTGCCAGCTATTTTCCCGCCGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAg < 1:650448/138‑1 (MQ=255) tccccccAACACCCGCCAATTTATCCACCCCCTTGTGCAAAAATGCCTCCGCGGCGGCTTTCTGCAAGCTATTTTCCCGCCAA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGc < 1:351866/132‑1 (MQ=255) ttATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGtt > 2:713034/1‑78 (MQ=255) ttATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGA‑AACAAAACCGCCGtt < 1:713034/78‑1 (MQ=255) tATCCAACGCGATGTAAAAAATGGCCTTGGGGGCGGCGTTCTCCCAGTTATTTTCCCGCCAC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCg < 1:226522/138‑1 (MQ=255) cccAACGCCTGGTGAAAAAATGCCTCCGCGGCGGTTGCCGGCCAGTTATTTTCCCGCCAA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgct < 2:264683/137‑1 (MQ=255) cAACCCAATGTGAAAAATTGCCTCGGCGGGGGTTTTCTGCCACTTATTTTCCCGCCAC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgct < 1:238661/139‑1 (MQ=255) cGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGGAAAAACATTTAACCCCCCCCT‑TTGTGTGGGAATAGCCGCTggg > 2:593107/1‑138 (MQ=25) gtgAAAAATTGCCTCCGGGGCGCCTGTCTGCCAGTTATTTTCCCGCCGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAt < 1:365598/139‑1 (MQ=255) gaaaaaaaaCCCCTCCGGGGCGTCTTCCTGCAAGCTTTTTCCCCCCCAA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAt < 2:7730/135‑1 (MQ=255) ttCTGCC‑GCTTTTTTTCCCCCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc < 1:763241/137‑1 (MQ=255) gCCAGTTTTTTTCCCGCCGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTc < 2:173686/139‑1 (MQ=255) | CGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACC‑GTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTC > NZ_CP009273/353464‑353717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |