| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,074,924 | 0 | A | G | 52.9% | 8.8 / 19.7 | 17 | D55G (GAT→GGT) | putP | sodium/proline symporter PutP |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base G (9/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATG‑GGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCG > NZ_CP009273/1074815‑1075053 | atatTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGGTATGGGGGGCTGGCTGTTTATG‑GGGGTgg > 1:65392/1‑138 (MQ=255) attgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATG‑GGGTTGCCGGgcg < 2:308723/139‑1 (MQ=255) attgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGACATTCGTGACGGCATTATCGGCGGGTGGGGGGGGTATGAGGGGCTGGCGGTTTATG‑GGGGTGCCGGgcg > 2:359140/1‑139 (MQ=255) ggCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGAGGGTGTGAGATGGGCGGCCGGGGGGTGATGTGGGTTG‑CGGGCGGTGTAttttttttcag > 2:490462/1‑135 (MQ=255) ggCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGAGGGGCGGGTATAAGAGGGTGGGGGTTTATA‑GGGGTGGCGGGCGCTGTtttttttttggg > 1:606542/1‑135 (MQ=255) aTCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGGATGGGGGGGTGGCTGTTTATG‑GGGGTGGCGGGGGGGGTTTTTTTTTTCgggggt > 1:315392/1‑136 (MQ=255) tatTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATG‑GGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGc < 2:106923/139‑1 (MQ=255) ttCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTAGCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATG‑GGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCt < 2:207683/139‑1 (MQ=255) ttCTGGGCGGTCGTAGTCTTGGGACATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATG‑GGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCt < 1:359140/139‑1 (MQ=255) ggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGGTATGAGGGGCTGGGTGTTTATG‑GGGGTGCCGGGCGCTGGTTTTTTTTTCGGGGTTTTCGAAAGGTGGATCGCCCTTGGCCTGGCAt > 1:615546/1‑139 (MQ=255) tCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGATAAGGGCGGCGGGGTGTTTAAG‑GGGGTTCCGGGGGCTGGTTTTTTTTTCGGGGGTTTTGTAAGCTGGATCGGCCTTGGCCGGGCATTAggg > 2:366981/1‑138 (MQ=255) tCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGGATGAGCGGCTGGGTGGTTATG‑GGGGTGCCGGGGGCTGTTTTTTTTTTCGGGGTTTTCGGAAACTGGGTCGCCCTTTGCCTGGCCTTAGgc > 1:132948/1‑139 (MQ=255) tAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATG‑GGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGcgcg < 2:315392/139‑1 (MQ=255) gggCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGGTATTAGGGGGCGGGTGTTTATG‑GGGGTGCCGGGGGCTGTTTTTTTTTTCCGGGTTTTCGGAAGCTGGGGCGCCCTTTGCCTGCCCTTTGGCGCGGGGGtt > 2:249217/1‑138 (MQ=255) gggCCATTCGTGACGGCATTATCGGCGGGTGCGGCGGGTATGAGCGGGTGGCTGTTTATG‑GGGGTGCCGGGGGCTGTTTTTTTTTTCGGGGTTTCCGAAAGCTGGGTCGCCCTTGGGCTGGCATTTGGGGCGGGGGtt > 1:301013/1‑138 (MQ=255) ttCGTGACGGCATTATCGGCGGGTGGGGCGGGTATGGGCGGGTGGCTGGTAATT‑GGGGTGTCGGGGGCTGGTTTTTTTTCCGGGGTTTTCGCAAGGTGGGTCGGCACTTGCCCGACATTATGCGCGGGGGTTAAATgg > 1:111709/1‑138 (MQ=255) gTGCGTCGGATATGAGCGGCTGGCTGTTAATG‑GGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCg < 2:65392/139‑1 (MQ=255) | ATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATG‑GGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCG > NZ_CP009273/1074815‑1075053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |