| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,875,427 | 0 | G | . | 73.5% | 62.7 / 37.1 | 34 | coding (640/924 nt) | dmlR | DNA‑binding transcriptional regulator DmlR |
| Reads supporting (aligned to +/- strand): ref base G (5/4); new base . (13/12); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTAAATGACCACTGGCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCATGGGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGGCGCGCCATGTTGCGCAATAAATTCCGGCGACGCGCA > NZ_CP009273/1875294‑1875562 | aCTAAATGACCACTGGCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCATGGGGGc < 1:515522/139‑1 (MQ=255) aaTGACCACTGGCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCcttct > 1:593539/1‑139 (MQ=255) ccACTGGCAATGTTTTCGCTAACATCACACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCATTCTGTAtt > 2:614484/1‑139 (MQ=255) cACTGGCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTg > 1:107079/1‑139 (MQ=255) ggCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCATGGGGGCCTTCTTTATTGCg > 1:22523/1‑138 (MQ=255) ggCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGt > 1:749017/1‑138 (MQ=255) tCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGa < 2:225097/138‑1 (MQ=255) tCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGa < 1:670337/138‑1 (MQ=255) aCATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCATGGGGGCCTTCTTTATTGCGTAATTGCCAGACAcc < 2:22523/137‑1 (MQ=255) aCATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCATGGGGGCCTTCTTTATTGCGTAATTGCCAGACACCaa < 1:619819/139‑1 (MQ=255) gAACGCAGCGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATcgcg > 1:400033/1‑139 (MQ=255) gAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATcgcg < 1:150921/139‑1 (MQ=255) gAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATcgcg < 2:107079/139‑1 (MQ=255) aCGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCATGGGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCgcgc < 2:637536/139‑1 (MQ=255) aCGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGct < 2:72386/139‑1 (MQ=255) caccacTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCATGGGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATg > 1:588728/1‑136 (MQ=255) aTGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTa < 1:425260/108‑1 (MQ=255) aTGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTa > 2:425260/1‑108 (MQ=255) gACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACgg > 1:63413/1‑119 (MQ=255) gACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACgg < 2:63413/119‑1 (MQ=255) cATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCg < 1:512784/137‑1 (MQ=255) aTTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGgcgtg < 2:400033/138‑3 (MQ=255) aTTAGACGACAGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGgcgcgc > 1:676135/1‑139 (MQ=255) aGTGGGCCGGTGACTTTAATCGCATGGGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGGCGCGCCATGTTgcg > 2:443065/1‑139 (MQ=255) aGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCg > 2:771964/1‑123 (MQ=255) aGTGGGCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCg < 1:771964/123‑1 (MQ=255) ggCCGGTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGGCGCGCCATGTTGCGCAAt > 1:783280/1‑138 (MQ=255) cGGTGACTTTAATCGCATGGGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGGCGCGCCATGTTGCGCAATaaa > 1:426570/1‑139 (MQ=255) gTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACAcc > 1:726784/1‑49 (MQ=255) gTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACAcc < 2:726784/49‑1 (MQ=255) gTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGGCGCGcc < 1:92356/121‑1 (MQ=255) gTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGGCGCGcc > 2:92356/1‑121 (MQ=255) gTGACTTTAATCGCAT‑GGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGGCGCGCCATGTTGCGCAATAAATTc > 1:473964/1‑139 (MQ=255) tGGGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGGCGCGCCATGTTGCGCAATAAATTCCGGCGACGCGCa > 2:425288/1‑137 (MQ=255) | ACTAAATGACCACTGGCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCCATGATTAGACGACAGTGGGCCGGTGACTTTAATCGCATGGGGGCCTTCTTTATTGCGTAATTGCCAGACACCAAAAGGATGATCGCGCTCTTTAATGACCAGGCACGGCAATGCAGAAAGATCGGTTAAATGTTTCGGCGCGCCATGTTGCGCAATAAATTCCGGCGACGCGCA > NZ_CP009273/1875294‑1875562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |