Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,061,1800AC67.5% 49.0 / 41.7 40V486G (GTT→GGT) yihQsulfoquinovosidase
Reads supporting (aligned to +/- strand):  ref base A (6/7);  new base C (14/13);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGATCTCGCCGAGCTTGCCCGTTTCTTCAA  >  NZ_CP009273/4061044‑4061315
                                                                                                                                        |                                                                                                                                       
ggTGAAGGCGCTGAAACCGCCCCAGCGCAGCAGCAGCTTTTTTCTGCGTTTCATCCCAAGCGGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAAcc                                                                                                                                       <  2:579762/138‑1 (MQ=255)
    cgggCGCAGAAACCGCACCAGCGCGGCAGCACCTTTTTGCGGCGCTTCATCCCAAACGGGGGGGTTAAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACg                                                                                                                                   <  2:762319/137‑1 (MQ=255)
          cTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGc                                                                                                                             >  2:426299/1‑139 (MQ=255)
                  gCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCAtcgctg                                                                                                                     >  1:762319/1‑136 (MQ=255)
                         cgcAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAGGCCATCGTCGAGACTc                                                                                                               >  2:790714/1‑138 (MQ=255)
                              cagcagcTTTTTGCTGCGCTTAATCCAAAAGGGGGGGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCg                                                                                                         <  2:12009/139‑1 (MQ=255)
                                           ctgcGTTTCATCACAAACGGGGGGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTcgc                                                                                            <  2:719800/139‑1 (MQ=255)
                                              cgcTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTcgcccg                                                                                         >  2:646416/1‑139 (MQ=255)
                                                cTTCATCACAAGCGGGGGGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTcgcccgcc                                                                                       <  2:194598/139‑1 (MQ=255)
                                                          aaCAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCAtgg                                                                             <  1:790714/139‑1 (MQ=255)
                                                               ggtggtGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTcgcc                                                                                           >  1:210571/1‑120 (MQ=255)
                                                               ggtggtGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTcgcc                                                                                           <  2:210571/120‑1 (MQ=255)
                                                               ggtggtGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAg                                                                        >  1:277300/1‑139 (MQ=255)
                                                               ggtggtGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAGGCCAt                                                                                                                          >  2:672958/1‑89 (MQ=255)
                                                               ggtggtGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAGGCCAt                                                                                                                          <  1:672958/89‑1 (MQ=255)
                                                                   gtttAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAgg                                                                                                                              <  2:818401/78‑1 (MQ=255)
                                                                   gtgtAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAgg                                                                                                                              >  1:818401/1‑81 (MQ=255)
                                                                         ccGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGc                                                              >  2:657380/1‑139 (MQ=255)
                                                                           gCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTa                                                            <  2:291583/139‑1 (MQ=255)
                                                                             cgaTGTCGCTATGGTGCAGGCCATGTCCGGTCATTGCGAGCGACAGCGCCGCCGGGACACCCGCCGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTAcc                                                          <  2:56172/137‑1 (MQ=255)
                                                                             cAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTAcc                                                          >  2:124983/1‑139 (MQ=255)
                                                                              aaTGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTcg                                                                                             >  2:285464/1‑103 (MQ=255)
                                                                              aaTGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTcg                                                                                             <  1:285464/103‑1 (MQ=255)
                                                                                             cAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTcgcc                                                                                           >  2:271329/1‑90 (MQ=255)
                                                                                             cAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTcgcc                                                                                           <  1:271329/90‑1 (MQ=255)
                                                                                                gCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATa                                       <  1:825793/139‑1 (MQ=255)
                                                                                                     gTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGa                                   >  1:357702/1‑138 (MQ=255)
                                                                                                     gTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGa                                   >  2:27051/1‑138 (MQ=255)
                                                                                                          gTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCAtggtgg                                                                          >  2:262406/1‑94 (MQ=255)
                                                                                                          gTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCAtggtgg                                                                          <  1:262406/94‑1 (MQ=255)
                                                                                                          gTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAggc                             >  2:849260/1‑138 (MQ=255)
                                                                                                          gTCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGa                             >  2:422750/1‑139 (MQ=255)
                                                                                                           tCATTGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGAt                            <  1:657380/139‑1 (MQ=255)
                                                                                                               tGCCAGCGACAGCGCCGCCGGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGATCTCg                        >  1:16295/1‑139 (MQ=255)
                                                                                                                     cGACAGCGCCGCCGGGACAACCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGATCTCGCCGAGc                  >  2:772942/1‑139 (MQ=255)
                                                                                                                           cgccgcCGGGACAACCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCcaca                                                                                   >  2:229932/1‑68 (MQ=255)
                                                                                                                           cgccgcCGGGACAACCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCcaca                                                                                   <  1:229932/68‑1 (MQ=255)
                                                                                                                             ccgccgGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGATCTCGCCGAGCTTGCCCGt          <  1:820043/139‑1 (MQ=255)
                                                                                                                             ccgccgGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGATCTCGCCGAGCTTGCCCGt          <  1:658353/139‑1 (MQ=255)
                                                                                                                              cgccgGGACACCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGATCTCGCCGAGCTTGCCCGtt         <  2:846564/139‑1 (MQ=255)
                                                                                                                                     acacCCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGATCTCGCCGAGCTTGCCCGTTTCTTCaa  <  2:853333/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CGTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACCGACGCCAGGCCATCGTCGAGACTCCAGTCGACGTTCTGGTCGCCCGCCCACATCATGGTGGAGTATTTCTGGCTACCGGTAGAACCGGCGCGCATAAAGAAAAGGATCTCGCCGAGCTTGCCCGTTTCTTCAA  >  NZ_CP009273/4061044‑4061315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑