| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,564,882 | A→C | intergenic (‑136/+20) | gadC ← / ← gadB | acid resistance gamma‑aminobutyrate antiporter GadC/glutamate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,564,882 | 0 | A | C | 95.2% | 66.0 / ‑2.9 | 21 | intergenic (‑136/+20) | gadC/gadB | acid resistance gamma‑aminobutyrate antiporter GadC/glutamate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (10/10); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 | |||||||||||
ATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTC > NZ_CP009273/1564750‑1565015 | atCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACcccgtt < 1:52967/138‑1 (MQ=255) cTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACcccgttaccgtt > 1:734230/1‑138 (MQ=255) tAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCccgttaccgtta > 1:828611/1‑138 (MQ=255) aaaCGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTaa < 1:536668/137‑1 (MQ=255) aaaCGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAAc < 2:79114/139‑1 (MQ=255) cGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGtt < 2:734230/139‑1 (MQ=255) cGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGc > 2:662197/1‑139 (MQ=255) cGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGc > 2:619953/1‑139 (MQ=255) cACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACcccgttaccgtt < 1:167976/112‑1 (MQ=255) cACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACcccgttaccgtt > 2:167976/1‑112 (MQ=255) ttttGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttctg < 1:624643/138‑1 (MQ=255) aaGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGt < 2:109053/110‑1 (MQ=255) aaGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGt > 1:109053/1‑110 (MQ=255) aaCAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGt < 2:341802/98‑1 (MQ=255) aaCAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGt > 1:341802/1‑98 (MQ=255) actatTTTCCGTTGCTCCTGCCCGTGACCCGCCTTTGGCCCTTGTCCCGCCCCGGGTTCGTTTCGGGCCCCCGTTCCCGTTCCCCGTTCTCCGGTCTGTTTCCCGCTGTTCTGTTGGGCAATCCCCTGCCGTTTCGGGt > 1:202612/3‑139 (MQ=255) tatTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGa > 2:186940/1‑139 (MQ=255) cAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAggg < 2:20327/139‑1 (MQ=255) tGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGGTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAgg > 1:456430/1‑139 (MQ=255) aGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTg > 2:160614/1‑139 (MQ=255) tGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGt < 2:828611/137‑1 (MQ=255) tCCCGAAACGGGTTCGGTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCaa < 2:456430/138‑1 (MQ=255) gAAACGGGTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGtt < 2:630003/139‑1 (MQ=255) ggTTCGTTTCGGGACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCa > 2:265515/1‑138 (MQ=255) ggACCCCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTc > 2:414946/1‑138 (MQ=255) | ATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTC > NZ_CP009273/1564750‑1565015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |