Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,578,845	A→G	D633D (GAT→GAC)	ydeP ←	acid resistance putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,578,845	0	A	G	100.0%	123.1 / NA	36	D633D (GAT→GAC)	ydeP	acid resistance putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/14);  total (22/14)

ATAAAGACCACATCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGATCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCGGCGAACTC  >  NZ_CP009273/1578717‑1578976                                                                                                                                 |                                                                                                                                    aTAAAGACCACATCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTaa                                                                                                                             >  2:330449/1‑137 (MQ=255)           caTCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCt                                                                                                                 >  2:334827/1‑139 (MQ=255)                 gTTGACCGAATACCCCTCGTTAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGtct                                                                                                                                  >  2:150384/1‑116 (MQ=255)                 gTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGtct                                                                                                                                  <  1:150384/116‑1 (MQ=255)                                cTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTAcc                                                                                            >  2:360876/1‑139 (MQ=255)                                        cGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTCCTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAACTTAGCCTTACCTGACGGCg                                                                                    >  2:358228/1‑139 (MQ=255)                                        cGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCg                                                                                    <  1:334827/139‑1 (MQ=255)                                          atcatcCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGACATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGt                                                                                   >  2:406081/1‑138 (MQ=255)                                                       taAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCttt                                                                     >  1:347442/1‑139 (MQ=255)                                                          aTCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTcag                                                                  <  2:347442/139‑1 (MQ=255)                                                                           aTCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTAt                                                     >  2:614902/1‑135 (MQ=255)                                                                           aTCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTAt                                                     <  1:614902/135‑1 (MQ=255)                                                                           aTCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAgg                                                 >  2:9312/1‑139 (MQ=255)                                                                             cGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGt                                                >  2:372156/1‑138 (MQ=255)                                                                                ggCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGa                                                                                         >  1:729229/1‑94 (MQ=255)                                                                                ggCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGa                                                                                         <  2:729229/94‑1 (MQ=255)                                                                                ggCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCg                                                                                    <  1:714329/99‑1 (MQ=255)                                                                                ggCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCg                                                                                    >  2:714329/1‑99 (MQ=255)                                                                                ggCTGCGTACTGTCGCCATGACCAGCTTACTCTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGaa                                             >  1:220701/1‑138 (MQ=255)                                                                                     cGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGc                                                                                                  <  2:331591/80‑1 (MQ=255)                                                                                     cGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGc                                                                                                  >  1:331591/1‑80 (MQ=255)                                                                                     cGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAaccac                                       >  2:509239/1‑139 (MQ=255)                                                                                     cGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAaccac                                       >  2:205833/1‑139 (MQ=255)                                                                                     cGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGCCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAaccac                                       >  2:643205/1‑139 (MQ=255)                                                                                            tCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGAt                                 >  2:823439/1‑138 (MQ=255)                                                                                                 aTGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGa                           <  1:330449/139‑1 (MQ=255)                                                                                                     ccAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTCCCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg                       <  2:305263/139‑1 (MQ=255)                                                                                                     ccAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg                       <  2:184005/139‑1 (MQ=255)                                                                                                     ccAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg                       <  1:575508/139‑1 (MQ=255)                                                                                                     ccAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg                       <  2:559114/139‑1 (MQ=255)                                                                                                     ccAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg                       <  1:643205/139‑1 (MQ=255)                                                                                                     ccAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg                       <  1:823439/139‑1 (MQ=255)                                                                                                        gCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTg                    >  1:370066/1‑139 (MQ=255)                                                                                                                  aaaCGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCg          >  2:523737/1‑139 (MQ=255)                                                                                                                   aaCGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCgg         >  2:559941/1‑139 (MQ=255)                                                                                                                          aaGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCGGCGAActc  >  1:328720/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                    ATAAAGACCACATCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGATCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCGGCGAACTC  >  NZ_CP009273/1578717‑1578976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑