Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,580,214	T→C	D177G (GAC→GGC)	ydeP ←	acid resistance putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,580,214	0	T	C	100.0%	91.3 / NA	27	D177G (GAC→GGC)	ydeP	acid resistance putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/11);  total (16/11)

GTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGG  >  NZ_CP009273/1580082‑1580314                                                                                                                                     |                                                                                                     gTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACCCCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGa                                                                                                 <  2:89459/138‑1 (MQ=255)       gggTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTAt                                                                                          >  1:385986/1‑139 (MQ=255)       gggTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTAt                                                                                          >  1:485677/1‑139 (MQ=255)            aTGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTc                                                                                     >  1:107944/1‑139 (MQ=255)             tGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTc                                                                                     <  2:756106/138‑1 (MQ=255)                  aaTGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGc                                                                                       >  1:288317/1‑131 (MQ=255)                  aaTGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGc                                                                                       <  2:288317/131‑1 (MQ=255)                   aTGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTAtt                                                                              >  2:641306/1‑139 (MQ=255)                    tGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTAtt                                                                              >  1:843936/1‑138 (MQ=255)                      cAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCAc                                                                           <  1:512274/139‑1 (MQ=255)                         aTGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACggg                                                                        <  2:365807/139‑1 (MQ=255)                                          ctcAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATaaag                                                        <  2:385986/138‑1 (MQ=255)                                          ctcAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATaaag                                                        >  1:91719/1‑138 (MQ=255)                                                    tCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCa                                                                            <  1:159518/108‑1 (MQ=255)                                                    tCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCa                                                                            >  2:159518/1‑108 (MQ=255)                                                    tCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCtt                                             >  2:267452/1‑139 (MQ=255)                                                                 tCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCg                                >  1:190861/1‑139 (MQ=255)                                                                       tACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCg                           <  2:585974/138‑1 (MQ=255)                                                                           tACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGta                      >  1:830060/1‑139 (MQ=255)                                                                           tACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGta                      >  1:385615/1‑139 (MQ=255)                                                                             cacCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCTCGAAGta                      >  2:477111/1‑137 (MQ=255)                                                                                   tACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAAtt               >  1:74153/1‑138 (MQ=255)                                                                                          tGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGaa                         <  2:95996/121‑1 (MQ=255)                                                                                          tGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGaa                         >  1:95996/1‑121 (MQ=255)                                                                                               aaCCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTggg  <  2:385615/139‑1 (MQ=255)                                                                                                                         aTGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCg                           <  2:353751/88‑1 (MQ=255)                                                                                                                         aTGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCg                           >  1:353751/1‑88 (MQ=255)                                                                                                                                     |                                                                                                     GTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGG  >  NZ_CP009273/1580082‑1580314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑