| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,610,406 | T→C | A129A (GCT→GCC) | yneK → | putative protein YneK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,610,406 | 0 | T | C | 80.8% | 47.3 / 10.7 | 26 | A129A (GCT→GCC) | yneK | putative protein YneK |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (10/11); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01 | |||||||||||
AGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACAC > NZ_CP009273/1610270‑1610542 | aGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGcccc < 1:598535/139‑1 (MQ=255) aaCTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGc < 2:507068/138‑1 (MQ=255) cTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTg < 1:561810/139‑1 (MQ=255) gAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTAttt > 2:517775/1‑137 (MQ=255) aaGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTAttt > 2:151958/1‑136 (MQ=255) aaGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGt < 2:745173/139‑1 (MQ=255) aaaaTATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGa < 1:625376/139‑1 (MQ=255) taataaATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCtttctgtttct > 2:262907/1‑139 (MQ=255) aaGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGAttt > 1:373801/1‑138 (MQ=255) atttatttAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGaa > 2:565775/1‑138 (MQ=255) taGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTc > 1:598903/1‑138 (MQ=255) taGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTc > 1:648466/1‑138 (MQ=255) aTAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCgtg < 2:386902/96‑1 (MQ=255) aTAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCgtg > 1:386902/1‑96 (MQ=255) tAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAAt > 2:548893/1‑138 (MQ=255) tAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATg < 1:517775/139‑1 (MQ=255) aTTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGAt < 1:151958/139‑1 (MQ=255) tgGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGtt < 1:521273/139‑1 (MQ=255) tgGAGAAATTAATATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGtt < 1:689805/139‑1 (MQ=255) aaTATTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCaa > 1:396356/1‑137 (MQ=255) atatTTTTTGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAag < 2:112587/139‑1 (MQ=255) ttttttGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAgc < 2:648466/139‑1 (MQ=255) ttttGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAgc > 1:610548/1‑137 (MQ=255) ttttGCGCCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAgc < 2:610548/137‑1 (MQ=255) cgcCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATa < 1:685676/137‑1 (MQ=255) cgcCCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATa > 2:685676/1‑137 (MQ=255) cTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATacac > 2:291795/1‑138 (MQ=255) | AGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACAC > NZ_CP009273/1610270‑1610542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |