Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,622,039	A→G	E89G (GAG→GGG)	ydfG →	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,622,039	0	A	G	100.0%	134.7 / NA	37	E89G (GAG→GGG)	ydfG	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/18);  total (19/18)

TATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGAGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATAT  >  NZ_CP009273/1621918‑1622159                                                                                                                          |                                                                                                                         tataTCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGc                                                                                                           <  1:414369/137‑1 (MQ=255)      cGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGtt                                                                                                    >  2:597298/1‑139 (MQ=255)      cGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGtt                                                                                                    >  1:493287/1‑139 (MQ=255)        cccAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTg                                                                                                   >  2:609906/1‑138 (MQ=255)        cccAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTg                                                                                                   >  2:402040/1‑138 (MQ=255)           aaCTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGa                                                                                               >  1:262769/1‑139 (MQ=255)           aaCTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGa                                                                                               >  2:702369/1‑139 (MQ=255)                gACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTgg                                                                                           >  2:52560/1‑138 (MQ=255)                              cgccgcTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGAtgattga                                                                             >  1:351666/1‑138 (MQ=255)                                  gcTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACca                                                                        <  1:589899/139‑1 (MQ=255)                                   cTATTGAAGAG‑TGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaac                                                                      <  2:212622/139‑1 (MQ=255)                                         aaGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaacaaca                                                                  <  2:262769/138‑1 (MQ=255)                                             gaagCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATAACCTGGTAAATAATGCCGCTCTGGCGGTGGGCATGGGGCCTGTGCATAAAGCCAGCGTTGCAGACTGGGAAACGATGATTGATACAAACAACAAAGGc                                                             >  2:381217/4‑139 (MQ=255)                                             gaTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                             <  1:394581/139‑1 (MQ=255)                                             gaTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                             <  1:702369/139‑1 (MQ=255)                                             gaTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                             <  1:484322/139‑1 (MQ=255)                                             gaTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                             <  1:864401/139‑1 (MQ=255)                                             gaTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                             <  2:232771/139‑1 (MQ=255)                                             gaTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                             <  1:402040/139‑1 (MQ=255)                                              aTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCa                                                                                                         <  1:496130/94‑1 (MQ=255)                                              aTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCa                                                                                                         >  2:496130/1‑94 (MQ=255)                                              aTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGcc                                                            >  1:397723/1‑139 (MQ=255)                                                gCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTg                                                          >  2:637110/1‑139 (MQ=255)                                                gCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTg                                                          >  2:305805/1‑139 (MQ=255)                                                          cTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGa                                                <  2:489125/139‑1 (MQ=255)                                                              cTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGCcgcg                                            <  2:397723/139‑1 (MQ=255)                                                                      tggtgCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCa                                                                                                         >  2:30810/1‑70 (MQ=255)                                                                      tggtgCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCa                                                                                                         >  2:503255/1‑70 (MQ=255)                                                                      tggtgCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCa                                                                                                         <  1:503255/70‑1 (MQ=255)                                                                      tggtgCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCa                                                                                                         <  1:30810/70‑1 (MQ=255)                                                                                ttGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTAt                          >  1:472603/1‑139 (MQ=255)                                                                                   atatCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                             >  2:334498/1‑101 (MQ=255)                                                                                   atatCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                             <  1:334498/101‑1 (MQ=255)                                                                                   atatCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGt                       >  1:242842/1‑139 (MQ=255)                                                                                               ataatGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCa           <  1:52560/139‑1 (MQ=255)                                                                                                     ccGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCa     <  2:472603/139‑1 (MQ=255)                                                                                                        gCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCatat  >  1:732742/1‑139 (MQ=255)                                                                                                                          |                                                                                                                         TATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGAGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATAT  >  NZ_CP009273/1621918‑1622159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑