| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,654,400 | T→C | F692S (TTC→TCC) | ynfE → | dimethyl sulfoxide reductase subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,654,400 | 0 | T | C | 97.1% | 119.2 / ‑3.6 | 34 | F692S (TTC→TCC) | ynfE | dimethyl sulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (16/17); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
GCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGG > NZ_CP009273/1654273‑1654531 | gCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACaa < 2:670613/137‑1 (MQ=255) cGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATc < 1:127182/139‑1 (MQ=255) cGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTactcact < 1:864718/139‑1 (MQ=255) cTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGAcc < 2:78111/139‑1 (MQ=255) cTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGAcc < 2:564466/139‑1 (MQ=255) cTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCa < 1:594091/139‑1 (MQ=255) ggAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCaa > 1:612851/1‑138 (MQ=255) aGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGAt < 2:255278/139‑1 (MQ=255) aGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGAt > 1:473520/1‑139 (MQ=255) aTTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcctgc > 1:67826/1‑139 (MQ=255) tGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTactcact > 1:296367/1‑99 (MQ=255) tGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTactcact < 2:296367/99‑1 (MQ=255) ccAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGt < 2:612851/138‑1 (MQ=255) gTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCag > 2:763419/1‑138 (MQ=255) gTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCag > 1:205902/1‑138 (MQ=255) tATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTact < 2:14588/87‑1 (MQ=255) tATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTact > 1:14588/1‑87 (MQ=255) aaCCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGAt < 2:118500/138‑1 (MQ=255) tGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTa > 1:426320/1‑64 (MQ=255) tGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTa < 2:426320/64‑1 (MQ=255) tGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGAt > 2:45803/1‑90 (MQ=255) tGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGAt < 1:45803/90‑1 (MQ=255) cccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtg > 1:629024/1‑112 (MQ=255) cccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtg < 2:629024/112‑1 (MQ=255) aaCGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAg > 1:714949/1‑123 (MQ=255) aaCGTAGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAg < 2:714949/123‑1 (MQ=255) aGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCagg < 1:534986/97‑1 (MQ=255) aGAACCTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCagg > 2:534986/1‑97 (MQ=255) cTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATc > 1:520143/1‑103 (MQ=255) cTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATc < 2:520143/103‑1 (MQ=255) cTTCCCACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAAc > 1:627670/1‑139 (MQ=255) accACTGCAACTGTTTGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGTTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGc > 2:603355/2‑139 (MQ=255) tttGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCggg > 1:481904/1‑139 (MQ=255) tttGGTTCCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCgg < 2:481904/138‑1 (MQ=255) | GCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGG > NZ_CP009273/1654273‑1654531 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |