Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,707,599	A→T	I192F (ATC→TTC)	dtpA →	dipeptide/tripeptide permease DtpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,707,599	0	A	T	100.0%	168.6 / NA	42	I192F (ATC→TTC)	dtpA	dipeptide/tripeptide permease DtpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/17);  total (25/17)

TGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGATCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGCCACCT  >  NZ_CP009273/1707475‑1707734                                                                                                                             |                                                                                                                                        tGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAAc                                                                                                                           >  2:776979/1‑139 (MQ=255)           aTGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCa                                                                                                                                      >  2:540515/1‑118 (MQ=255)           aTGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCa                                                                                                                                      <  1:540515/118‑1 (MQ=255)           aTGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCt                                                                                                                 >  2:639650/1‑139 (MQ=255)               actacATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGcc                                                                                                              >  2:793638/1‑138 (MQ=255)                   cATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          <  1:124930/138‑1 (MQ=255)                   cATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          <  2:638346/138‑1 (MQ=255)                   cATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          <  1:854073/138‑1 (MQ=255)                      gTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTgg                                                                                                      >  2:380152/1‑139 (MQ=255)                      gTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTgg                                                                                                      >  2:762598/1‑139 (MQ=255)                        ccGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          >  1:10778/1‑133 (MQ=255)                        ccGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          <  2:10778/133‑1 (MQ=255)                        ccGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGt                                                                                                    >  1:376527/1‑139 (MQ=255)                        ccGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGt                                                                                                    >  2:756068/1‑139 (MQ=255)                        ccGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGt                                                                                                    >  2:840336/1‑139 (MQ=255)                        ccGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGt                                                                                                    >  2:88354/1‑139 (MQ=255)                        ccGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGt                                                                                                    >  2:566017/1‑139 (MQ=255)                             aaCATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTaaa                                                                                                <  2:365477/138‑1 (MQ=255)                               cATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAg                                                                                             >  2:352517/1‑139 (MQ=255)                                 tCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTa                                                                                           <  1:639650/139‑1 (MQ=255)                                   ggCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTAc                                                                                          <  1:475528/138‑1 (MQ=255)                                          tcttctCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCaa                                                                                   <  1:88354/138‑1 (MQ=255)                                                         cTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGc                                                                    <  1:380152/138‑1 (MQ=255)                                                           aCGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGGGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCCAAACCAGACTTCGAGCCTa                                                                 >  2:343380/1‑139 (MQ=255)                                                           aCGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTa                                                                 >  2:416215/1‑139 (MQ=255)                                                               cGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCaa                                                             <  1:851916/139‑1 (MQ=255)                                                                   gctggcCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTAc                                                         >  2:94041/1‑139 (MQ=255)                                                                        ccGCGAAATACGGCTGGTGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGTCTAGCAACTACCGTaa                                                    >  2:174502/1‑139 (MQ=255)                                                                                tACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACctgctgc                                            <  1:322395/139‑1 (MQ=255)                                                                                             ttgcgtttgcgtTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCa                                                                              >  2:492960/1‑92 (MQ=255)                                                                                             ttgcgtttgcgtTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCa                                                                              <  1:492960/92‑1 (MQ=255)                                                                                             ttgcgtttgcgtTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTgg                               >  1:737038/1‑139 (MQ=255)                                                                                             ttgcgtttgcgtTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTgg                               <  1:352517/139‑1 (MQ=255)                                                                                                     gcgttGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACctgctgct                                           >  2:560694/1‑119 (MQ=255)                                                                                                     gcgttGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACctgctgct                                           <  1:560694/119‑1 (MQ=255)                                                                                                             cGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATc               <  2:246419/139‑1 (MQ=255)                                                                                                                          ctgTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACctgctg                                             <  2:154887/96‑1 (MQ=255)                                                                                                                          ctgTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACctgctg                                             >  1:154887/1‑96 (MQ=255)                                                                                                                          ctgTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGCCACCt  >  1:45661/1‑139 (MQ=255)                                                                                                                          ctgTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGCCACCt  >  2:795153/1‑139 (MQ=255)                                                                                                                          ctgTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGCCACCt  >  2:801943/1‑139 (MQ=255)                                                                                                                          ctgTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGCCACCt  >  1:226028/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                        TGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGATCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGCCACCT  >  NZ_CP009273/1707475‑1707734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑