| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,708,538 | A→G | intergenic (+10/‑96) | dtpA → / → gstA | dipeptide/tripeptide permease DtpA/glutathione transferase GstA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,708,538 | 0 | A | G | 94.7% | 47.1 / ‑3.2 | 19 | intergenic (+10/‑96) | dtpA/gstA | dipeptide/tripeptide permease DtpA/glutathione transferase GstA |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (7/11); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
CGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGT > NZ_CP009273/1708402‑1708657 | cgTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGgaa > 2:766550/1‑139 (MQ=255)cgTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGgaa > 2:371854/1‑139 (MQ=255) tGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCg > 2:392825/1‑139 (MQ=255) cTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACttt < 1:638466/139‑1 (MQ=255) cGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAg < 1:766550/139‑1 (MQ=255) gCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttt > 2:210577/1‑139 (MQ=255) aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGtt > 2:654869/1‑139 (MQ=255) gACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACg > 1:186340/1‑139 (MQ=255) ccGCGCCGAAACTGCCCCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCa < 1:210577/139‑1 (MQ=255) cgcgCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTTAGCAACGccc > 1:62471/1‑137 (MQ=255) ccGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTAcc < 1:751516/139‑1 (MQ=255) gCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAACCAGCCGTGGCGTAAATTTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTa < 2:88771/139‑1 (MQ=255) gCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca < 1:721906/139‑1 (MQ=255) cATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca > 2:310707/1‑138 (MQ=255) aTGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca < 2:1272/139‑1 (MQ=255) ggACGCTGCAGAAAAAGGGGCGAAAGCAGCCGGAGCGTAAAATTCGGGGGACCTCTTTTCAAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAg < 2:62471/138‑1 (MQ=255) cGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACg > 2:386876/1‑103 (MQ=255) cGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACg < 1:386876/103‑1 (MQ=255) cAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAt > 2:439971/1‑139 (MQ=255) agcCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCg < 1:145165/139‑1 (MQ=255) cGTAGCGAAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGt < 1:310707/139‑1 (MQ=255) | CGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGT > NZ_CP009273/1708402‑1708657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |