| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,713,741 | A→G | I40T (ATC→ACC) | anmK ← | anhydro‑N‑acetylmuramic acid kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,713,741 | 0 | A | G | 78.6% | 50.7 / 11.4 | 28 | I40T (ATC→ACC) | anmK | anhydro‑N‑acetylmuramic acid kinase |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base G (10/12); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTGGTGCCTGACATAACGCCAATAAAGCGGCCCGATTTCATAGTTCATCCT > NZ_CP009273/1713616‑1713869 | tAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCa > 2:698275/1‑138 (MQ=255) gCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCaaa < 1:94553/132‑1 (MQ=255) gCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCaaa > 2:94553/1‑132 (MQ=255) tGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCtg > 2:693009/1‑139 (MQ=255) aCCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCtgtg > 2:487030/1‑139 (MQ=255) ttGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGcc < 1:774590/139‑1 (MQ=255) tATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCcaacac < 1:487030/139‑1 (MQ=255) tATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCcaacac < 1:693009/139‑1 (MQ=255) tATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCcaacac < 1:698275/139‑1 (MQ=255) aGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGc < 2:844004/95‑1 (MQ=255) aGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGc > 1:844004/1‑95 (MQ=255) tCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCaa < 1:262729/137‑1 (MQ=255) tCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAaca > 1:70488/1‑139 (MQ=255) tCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAac < 1:98432/138‑1 (MQ=255) tCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAac < 1:639540/138‑1 (MQ=255) gAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGc > 1:437892/1‑139 (MQ=255) aaGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTg < 2:119861/139‑1 (MQ=255) tAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTGGTGcc < 2:856362/139‑1 (MQ=255) tgGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTGGTGCCTGACATaa > 1:550423/1‑139 (MQ=255) ggCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGGTCGGCCAACTCAAACTTGCCAACTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTGGTGCCTGACATAACGCCaa > 2:765222/1‑138 (MQ=255) aGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCa > 1:449575/1‑49 (MQ=255) aGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCa < 2:449575/49‑1 (MQ=255) aGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTGGTGCCTGACATAACGCCAATAAAGCGGccc > 2:305717/1‑138 (MQ=255) aGCCTGTTTCAGAGATACCGGGATCGGCCAACTCa < 1:586922/35‑1 (MQ=255) aGCCTGTTTCAGAGATACCGGGATCGGCCAACTCa > 2:586922/1‑35 (MQ=255) agaTACCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTGGTGCCTGACATAACGCCAATAAAGCGGCCCGATTTCATAGTTCATCCt > 1:55519/1‑139 (MQ=255) aCCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTGGTGCCt < 2:351637/93‑1 (MQ=255) aCCGGGGTCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTGGTGCCt > 1:351637/1‑93 (MQ=255) | TAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTACAGCCTGTTTCAGAGATACCGGGATCGGCCAACTCAAACTTGCCAGCTGTGCGACCCGGTGTTCATCAATTGTCGCCAACACAACATCAACACCATCAAGGCTGGTGCCTGACATAACGCCAATAAAGCGGCCCGATTTCATAGTTCATCCT > NZ_CP009273/1713616‑1713869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |