Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	204,632	T→C	V1007A (GTC→GCC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	204,632	0	T	C	100.0%	152.2 / NA	44	V1007A (GTC→GCC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/20);  total (24/20)

ACCGGACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCA  >  NZ_CP009273/204496‑204751                                                                                                                                         |                                                                                                                        aCCGGACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCa                                                                                                                       >  2:804361/1‑139 (MQ=255) aCCGGACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCa                                                                                                                       >  2:571226/1‑139 (MQ=255) aCCGGACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCa                                                                                                                       >  1:666115/1‑139 (MQ=255)     gACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCAcc                                                                                                                     >  2:551660/1‑137 (MQ=255)     gACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCAcc                                                                                                                     >  1:32745/1‑137 (MQ=255)            tATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCgg                                                                                                             >  2:64891/1‑138 (MQ=255)            tATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCgg                                                                                                             >  1:691833/1‑138 (MQ=255)                   cAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCGCGTTGTTGCCGCGCGGGTGATGGCCACCAAGCGCGGCAAGCGTa                                                                                                     <  2:407785/139‑1 (MQ=255)                    aGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTAt                                                                                                    >  2:511414/1‑139 (MQ=255)                    aGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTAt                                                                                                    >  2:242432/1‑139 (MQ=255)                    aGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTAt                                                                                                    >  1:805219/1‑139 (MQ=255)                             aaGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTg                                                                                           >  1:782375/1‑139 (MQ=255)                                 gaTTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACg                                                                                       <  1:242432/139‑1 (MQ=255)                                  aTTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGc                                                                                      >  2:671586/1‑139 (MQ=255)                                                  gAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGt                                                                                                      <  2:749832/107‑1 (MQ=255)                                                  gAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGt                                                                                                      >  1:749832/1‑107 (MQ=255)                                                     gCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGggcg                                                                   >  2:637899/1‑139 (MQ=255)                                                                  aGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGAtg                                                      <  1:671586/139‑1 (MQ=255)                                                                  aGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGAtg                                                      <  2:666115/139‑1 (MQ=255)                                                                   gACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGAtgt                                                     <  1:637899/139‑1 (MQ=255)                                                                      aTGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGAtgttgt                                                  >  2:611284/1‑139 (MQ=255)                                                                            ccGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTg                                            <  1:571226/139‑1 (MQ=255)                                                                                 aGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGcc                                       <  1:64891/139‑1 (MQ=255)                                                                                  gAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGccc                                      <  2:771450/139‑1 (MQ=255)                                                                                  gAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGccc                                      <  2:280482/139‑1 (MQ=255)                                                                                  gAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGccc                                      <  1:110945/139‑1 (MQ=255)                                                                                  gAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGccc                                      <  2:32745/139‑1 (MQ=255)                                                                                   aaCGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCt                                     >  1:843347/1‑139 (MQ=255)                                                                                        ggTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGAt                                 >  1:284154/1‑138 (MQ=255)                                                                                                  tcaCGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCggg                                                                     <  2:757803/92‑1 (MQ=255)                                                                                                  tcaCGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCggg                                                                     >  1:757803/1‑92 (MQ=255)                                                                                                  tcaCGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGc                       >  2:581393/1‑138 (MQ=255)                                                                                                    aCGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGa                                           <  1:470471/116‑1 (MQ=255)                                                                                                    aCGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGa                                           >  2:470471/1‑116 (MQ=255)                                                                                                       gCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGc                 <  2:284154/139‑1 (MQ=255)                                                                                                        cTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCt                >  2:784910/1‑139 (MQ=255)                                                                                                                 atacgattgCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGa       >  1:374502/7‑139 (MQ=255)                                                                                                                  cGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaaaa          <  2:220668/135‑1 (MQ=255)                                                                                                                  cGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaaaa          >  1:220668/1‑135 (MQ=255)                                                                                                                   gttgttGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGa       <  1:511414/137‑1 (MQ=255)                                                                                                                    ttgttgCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCg    <  1:551660/139‑1 (MQ=255)                                                                                                                       ttgCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCa  <  1:611284/138‑1 (MQ=255)                                                                                                                        tgCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCa                                                                                         <  2:291587/50‑1 (MQ=255)                                                                                                                        tgCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCa                                                                                         >  1:291587/1‑50 (MQ=255)                                                                                                                                         |                                                                                                                        ACCGGACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCA  >  NZ_CP009273/204496‑204751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑