| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,765,154 | A→G | N95D (AAT→GAT) | ydiL → | YdiL family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,765,154 | 0 | A | G | 96.8% | 99.3 / ‑3.3 | 31 | N95D (AAT→GAT) | ydiL | YdiL family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (16/14); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01 | |||||||||||
TGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACACAA > NZ_CP009273/1765032‑1765286 | tGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTgg > 2:417117/1‑137 (MQ=255) gCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTAt < 2:839109/139‑1 (MQ=255) aaaaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGt < 1:737618/139‑1 (MQ=255) aaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAg < 1:516543/139‑1 (MQ=255) cATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgctg < 1:642682/139‑1 (MQ=255) catAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGca > 1:94430/1‑139 (MQ=255) tAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGcaca < 2:121085/139‑1 (MQ=255) aataaataACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTg > 1:205116/1‑139 (MQ=255) aataaataACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTg > 1:779478/1‑139 (MQ=255) aTGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGa > 1:66039/1‑138 (MQ=255) ttttttCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGaa < 1:461676/139‑1 (MQ=255) tttttCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAAt > 1:316744/1‑139 (MQ=255) cccGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCgt < 2:316744/139‑1 (MQ=255) cTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTa < 2:705778/139‑1 (MQ=255) cATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAg < 2:316413/89‑1 (MQ=255) cATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAg > 1:599573/1‑89 (MQ=255) cATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAg < 2:599573/89‑1 (MQ=255) cATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAg > 1:316413/1‑89 (MQ=255) aaCGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGaa < 1:417117/138‑1 (MQ=255) tCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGt < 2:853133/46‑1 (MQ=255) tCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGt > 1:853133/1‑46 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGCATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACacaa > 1:441209/1‑138 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCca > 2:271197/1‑88 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCca < 1:271197/88‑1 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCATAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACacaa > 2:427143/1‑138 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTacaac < 2:246365/114‑1 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTacaac > 1:246365/1‑114 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACacaa > 1:640251/1‑138 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACacaa > 2:530029/1‑138 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACacaa > 1:369654/1‑138 (MQ=255) aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACacaa < 2:794998/138‑1 (MQ=255) | TGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACACAA > NZ_CP009273/1765032‑1765286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |