| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,769,436 | A→G | D165G (GAC→GGC) | aroD → | type I 3‑dehydroquinate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,769,436 | 0 | A | G | 98.0% | 167.2 / ‑4.8 | 50 | D165G (GAC→GGC) | aroD | type I 3‑dehydroquinate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (20/29); total (20/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGACGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCAATTATCACGATGTCGATGGCAAAAAC > NZ_CP009273/1769304‑1769565 | atgatCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGa < 2:757132/139‑1 (MQ=255) tgatCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGat < 2:287160/139‑1 (MQ=255) tgatCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGat < 1:596219/139‑1 (MQ=255) ggTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCt < 1:843167/139‑1 (MQ=255) ccGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCt > 1:541639/1‑139 (MQ=255) ccGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCCTCGGCGCCGATATTCCTAAGATTGCGCt > 1:786065/1‑139 (MQ=255) gTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGa < 2:541639/139‑1 (MQ=255) tCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGAt > 2:569737/1‑139 (MQ=255) cACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTa < 1:590605/139‑1 (MQ=255) cgcgCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTAc > 2:408859/1‑138 (MQ=255) agtagtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGat > 1:256139/1‑91 (MQ=255) agtagtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGat < 2:256139/91‑1 (MQ=255) gtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGttgc > 2:753890/1‑139 (MQ=255) gtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGttgc > 1:421117/1‑139 (MQ=255) gtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGttg < 2:10198/138‑1 (MQ=255) gTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCG‑TGTGCTGACGTTGCTTGCcg > 2:602452/1‑139 (MQ=255) tCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCTGCGCCGATATTCCTAAGATTGCGCTGAGGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCcg < 1:607164/139‑1 (MQ=255) tCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCg > 1:727113/1‑79 (MQ=255) tCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCg < 2:727113/79‑1 (MQ=255) tCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCcg < 1:430750/139‑1 (MQ=255) tCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCcg > 1:421451/1‑139 (MQ=255) tCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCcg < 1:372130/139‑1 (MQ=255) aCCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTa > 2:763687/1‑108 (MQ=255) aCCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTa < 1:763687/108‑1 (MQ=255) ccATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGAcc > 2:637048/1‑139 (MQ=255) gACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTcc > 1:315928/1‑77 (MQ=255) gACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTcc < 2:315928/77‑1 (MQ=255) cATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATg > 2:576573/1‑138 (MQ=255) tAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAg < 1:780362/139‑1 (MQ=255) tAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAg < 1:234733/139‑1 (MQ=255) aaCGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAg < 1:462573/139‑1 (MQ=255) aCGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGc < 1:365676/139‑1 (MQ=255) aCGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGc < 2:421451/139‑1 (MQ=255) aCGCCGGAAGCCGAAGAAATCAATGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGc < 2:355990/139‑1 (MQ=255) cGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGat > 2:812382/1‑59 (MQ=255) cGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGat < 1:812382/59‑1 (MQ=255) cGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGACGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCa < 2:451446/139‑1 (MQ=255) aagaaATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCg < 1:586623/139‑1 (MQ=255) aTCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGa < 2:112944/131‑1 (MQ=255) aTCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGa > 1:112944/1‑131 (MQ=255) aTTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCG‑TGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCAATTa < 1:602452/138‑1 (MQ=255) tCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGCTCGTCCAATTATCACGATg > 1:365306/1‑139 (MQ=255) cgcAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCcg > 1:603278/1‑85 (MQ=255) cgcAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCcg < 2:603278/85‑1 (MQ=255) cgcAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCAATTATCACGATGTCg < 1:637048/138‑1 (MQ=255) tGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCAATTATCACGATGTCGATGGCaaa < 1:569737/139‑1 (MQ=255) aaTCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCAATTATCACGATGTCGATGGCAAAAAc < 1:408859/139‑1 (MQ=255) aaTCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCAATTATCACGATGTCGATGGCAAAAAc > 2:807316/1‑139 (MQ=255) ttCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAg > 2:198248/1‑87 (MQ=255) ttCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAg < 1:198248/87‑1 (MQ=255) | ATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGACGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCAATTATCACGATGTCGATGGCAAAAAC > NZ_CP009273/1769304‑1769565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |