Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,776,037	T→G	V129G (GTT→GGT)	ydiS →	FAD‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,776,037	0	T	G	100.0%	140.8 / NA	41	V129G (GTT→GGT)	ydiS	FAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/17);  total (24/17)

CGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCC  >  NZ_CP009273/1775906‑1776172                                                                                                                                    |                                                                                                                                        cGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTc                                                                                                                                  >  1:528802/1‑139 (MQ=255)  gTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCg                                                                                                                                 <  2:2837/139‑1 (MQ=255)                         gcagcCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAAc                                                                                                          <  2:579803/139‑1 (MQ=255)                                    ttCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTg                                                                                                <  2:136782/138‑1 (MQ=255)                                                           gTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGGTCGTGAAGGAAACAAGGGCACTGGCGGGCAGGCTGGGGATGGTAttt                                                                        >  2:417337/1‑138 (MQ=255)                                                            tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtct                                                                       >  2:829209/1‑139 (MQ=255)                                                            tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtct                                                                       >  1:33353/1‑139 (MQ=255)                                                            tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtct                                                                       <  2:528802/139‑1 (MQ=255)                                                            tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtct                                                                       >  1:446914/1‑139 (MQ=255)                                                            tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtct                                                                       >  1:734286/1‑139 (MQ=255)                                                            tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAACCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACCCGCTGGTTCGGGAAGGCAACAAGGGCACTGGCGTGCAGGCTGGGGGTGATATtct                                                                       >  2:102671/1‑139 (MQ=255)                                                             aTTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTGGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtctc                                                                      >  1:200784/1‑139 (MQ=255)                                                              ttGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtctc                                                                      >  1:725875/1‑138 (MQ=255)                                                                 cGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCCGGCTGGGGATGATATTCTCGAAg                                                                  >  2:561563/1‑139 (MQ=255)                                                                 cGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAg                                                                  >  2:445572/1‑139 (MQ=255)                                                                   tAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGc                                                                 >  1:613167/1‑138 (MQ=255)                                                                     aTCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGaa                                                              <  2:725875/139‑1 (MQ=255)                                                                      tCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTg                                                                                                >  1:70393/1‑104 (MQ=255)                                                                      tCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTg                                                                                                <  2:70393/104‑1 (MQ=255)                                                                      tCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGCCGACGCGCTGGTTCGTGAAGGGAACAAGGGCACTGGCGCGCAGGCTGGGGCTGATATTCTCGAAGCGAAt                                                             >  2:445614/1‑139 (MQ=255)                                                                           tGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATgtggt                                                        >  1:417707/1‑139 (MQ=255)                                                                                   tGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGa                                                               >  2:539610/1‑124 (MQ=255)                                                                                   tGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGa                                                               <  1:539610/124‑1 (MQ=255)                                                                                       tGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGa                                            >  1:102906/1‑139 (MQ=255)                                                                                        gATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGAt                                           >  2:428407/1‑139 (MQ=255)                                                                                         aTGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGAt                                           <  1:642655/138‑1 (MQ=255)                                                                                                aaGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTaa                                   >  1:826946/1‑139 (MQ=255)                                                                                                  gCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACt                                 <  2:417707/139‑1 (MQ=255)                                                                                                  gCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACt                                 <  1:626458/139‑1 (MQ=255)                                                                                                      aGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGAt                             >  1:91464/1‑139 (MQ=255)                                                                                                           gcgggcgCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACt                                 <  2:715962/130‑1 (MQ=255)                                                                                                           gcgggcgCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACt                                 >  1:715962/1‑130 (MQ=255)                                                                                                             gggcgCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGc                      <  2:33353/139‑1 (MQ=255)                                                                                                                  cgcAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCcgctc                 <  1:829209/139‑1 (MQ=255)                                                                                                                  cgcAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCcgctc                 >  1:251568/1‑139 (MQ=255)                                                                                                                         tATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTggg           >  1:496857/1‑138 (MQ=255)                                                                                                                          aTCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGGGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGa          <  2:446914/138‑1 (MQ=255)                                                                                                                               aGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGtt    >  2:407803/1‑139 (MQ=255)                                                                                                                                 gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc  <  1:428407/139‑1 (MQ=255)                                                                                                                                 gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc  <  2:613167/139‑1 (MQ=255)                                                                                                                                 gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc  <  1:63145/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        CGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCC  >  NZ_CP009273/1775906‑1776172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑