Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,809,188	T→C	D355D (GAT→GAC)	katE →	catalase HPII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,809,188	0	T	C	100.0%	63.3 / NA	19	D355D (GAT→GAC)	katE	catalase HPII
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/12);  total (7/12)

CCGGACGTGACCCGGACTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGATCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCAACCCGGATAACTTCTTTGCTGAAAACGAACAGGCGGCTTTC  >  NZ_CP009273/1809055‑1809296                                                                                                                                      |                                                                                                             ccGGACGTGACCCGGACTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACccaac                                                                                                         <  1:192694/139‑1 (MQ=255) ccGGACGTGACCCGGACTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACccaac                                                                                                         <  2:836354/139‑1 (MQ=255)                      aCCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACt                                                                                    <  2:639165/139‑1 (MQ=255)                          ccgcGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTg                                                                                >  2:353969/1‑139 (MQ=255)                                    tgGGAAGCCATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGc                                                                      >  2:446435/1‑139 (MQ=255)                                        aaGCCATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCgtgt                                                                  <  2:298740/139‑1 (MQ=255)                                           ccATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTAt                                                                                                >  1:469784/1‑106 (MQ=255)                                           ccATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTAt                                                                                                <  2:469784/106‑1 (MQ=255)                                              ttGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCa                                                             >  1:832247/1‑138 (MQ=255)                                                                cGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCa                                           <  2:134608/138‑1 (MQ=255)                                                                             gggCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCt                                                   >  1:818820/1‑117 (MQ=255)                                                                             gggCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCt                                                   <  2:818820/117‑1 (MQ=255)                                                                                   ccAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCAACCCGGATAACTTCTTTGc                        <  2:795059/138‑1 (MQ=255)                                                                                                 aagaagATGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCAACCCGGATAACTTCTTTGCTGAAAACGAACAgg          <  1:446435/138‑1 (MQ=255)                                                                                                       aTGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCAACCCGGATAACTTCTTTGCTGAAAACGAACAGGCGGCttt   <  2:775241/139‑1 (MQ=255)                                                                                                       aTGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCAACCCGGATAACTTCTTTGCTGAAAACGAACAGGCGGCttt   <  1:123302/139‑1 (MQ=255)                                                                                                        tGAATTCAAGTTCGACTTCGATCTTCTCGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCAACCCGGATAACTTCTTTGCTGAAAACGAACAGGCGGCTTTc  >  2:122532/1‑139 (MQ=255)                                                                                                                                 ctcGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCAACCCGGAt                                   <  1:521276/81‑1 (MQ=255)                                                                                                                                 ctcGACCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCAACCCGGAt                                   >  2:521276/1‑81 (MQ=255)                                                                                                                                      |                                                                                                             CCGGACGTGACCCGGACTTCCACCGCCGCGAGTTGTGGGAAGCCATTGAAGCAGGCGATTTTCCGGAATACGAACTGGGCTTCCAGTTGATTCCTGAAGAAGATGAATTCAAGTTCGACTTCGATCTTCTCGATCCAACCAAACTTATCCCGGAAGAACTGGTGCCCGTTCAGCGTGTCGGCAAAATGGTGCTCAATCGCAACCCGGATAACTTCTTTGCTGAAAACGAACAGGCGGCTTTC  >  NZ_CP009273/1809055‑1809296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑