Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,809,395	T→G	G424G (GGT→GGG)	katE →	catalase HPII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,809,395	0	T	G	100.0%	110.1 / NA	30	G424G (GGT→GGG)	katE	catalase HPII
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/14);  total (16/14)

AAAACGAACAGGCGGCTTTCCATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGA  >  NZ_CP009273/1809277‑1809516                                                                                                                       |                                                                                                                          aaaaCGAACAGGCGGCTTTCCATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGAt                                                                                                       >  1:457325/1‑139 (MQ=255)   aaCGAACAGGCGGCTTTCCATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTc                                                                                                     >  2:714229/1‑139 (MQ=255)        aCAGGCGGCTTTCCATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTCGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGAt                                                                                                 >  1:91214/1‑138 (MQ=255)                            ggCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTa                                                                            <  1:1989/139‑1 (MQ=255)                                 atCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCAt                                                                        >  1:590898/1‑138 (MQ=255)                                       ccGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTc                                                                  >  1:798767/1‑138 (MQ=255)                                             ctggactTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTg                                                           <  1:705668/139‑1 (MQ=255)                                                gactTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACg                                                        >  1:446162/1‑139 (MQ=255)                                                     cACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGgcat                                                    <  1:353969/138‑1 (MQ=255)                                                       ccAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGgcatgca                                                 <  1:411552/139‑1 (MQ=255)                                                            gATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCa                                            <  2:832247/139‑1 (MQ=255)                                                            gATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCa                                            <  1:714229/139‑1 (MQ=255)                                                                     ttGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGAt                                     <  2:781136/137‑1 (MQ=255)                                                                     ttGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGAt                                     <  2:590898/137‑1 (MQ=255)                                                                       gCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGa                                  <  1:216897/138‑1 (MQ=255)                                                                          gggACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGacac                               >  1:828150/1‑138 (MQ=255)                                                                             aCGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAAc                           >  1:364437/1‑139 (MQ=255)                                                                               gTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAAccc                         >  1:186029/1‑139 (MQ=255)                                                                                            taCCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATg                                          >  1:860455/1‑109 (MQ=255)                                                                                            taCCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATg                                          <  2:860455/109‑1 (MQ=255)                                                                                                 aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGAt                                     <  2:43330/109‑1 (MQ=255)                                                                                                 aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGAt                                     >  1:43330/1‑109 (MQ=255)                                                                                                 aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa       >  1:315648/1‑139 (MQ=255)                                                                                                 aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa       <  2:364437/139‑1 (MQ=255)                                                                                                 aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa       >  2:497965/1‑139 (MQ=255)                                                                                                 aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa       >  1:504089/1‑139 (MQ=255)                                                                                                   acacAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGAt                                     >  1:228181/1‑107 (MQ=255)                                                                                                   acacAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGAt                                     <  2:228181/107‑1 (MQ=255)                                                                                                   acacAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACt     >  1:389934/1‑139 (MQ=255)                                                                                                      caAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGa  <  1:497965/139‑1 (MQ=255)                                                                                                                       |                                                                                                                          AAAACGAACAGGCGGCTTTCCATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGA  >  NZ_CP009273/1809277‑1809516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑