Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,810,989	A→G	F135S (TTC→TCC)	chbG ←	chitin disaccharide deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,810,989	0	A	G	100.0%	89.0 / NA	26	F135S (TTC→TCC)	chbG	chitin disaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

CACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTC  >  NZ_CP009273/1810855‑1811122                                                                                                                                       |                                                                                                                                      cACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAAt                                                                                                                                   >  2:152340/1‑139 (MQ=255)           ttCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCggg                                                                                                                            <  2:380750/136‑1 (MQ=255)              aCCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACata                                                                                                                      <  1:678586/139‑1 (MQ=255)                cGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATg                                                                                                                    >  2:464108/1‑139 (MQ=255)                cGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATg                                                                                                                    <  1:567674/139‑1 (MQ=255)                  tAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGca                                                                                                                  <  1:465830/139‑1 (MQ=255)                           ctgctgAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACatgatgat                                                                                                         <  1:464108/139‑1 (MQ=255)                                   tCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCa                                                                                                 <  2:334378/139‑1 (MQ=255)                                     ccTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAg                                                                                               <  1:345486/139‑1 (MQ=255)                                     ccTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAg                                                                                               <  1:40258/139‑1 (MQ=255)                                              tGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAgg                                                                                      >  1:155625/1‑139 (MQ=255)                                              tGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAgg                                                                                      >  2:448389/1‑139 (MQ=255)                                              tGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAgg                                                                                      >  2:437113/1‑139 (MQ=255)                                              tGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAgg                                                                                      >  1:507942/1‑139 (MQ=255)                                              tGCGCAGGTTGACCGGCAAATCAAACACCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGATATCTGCGGGAACATATGTACATGATGATGGCTATCAAGCTGCGTAgg                                                                                      >  2:290061/1‑139 (MQ=255)                                                cgcAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGtt                                                                                    <  2:804075/139‑1 (MQ=255)                                                cgcAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGtt                                                                                    >  1:27403/1‑139 (MQ=255)                                                        gACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTcc                                                                             >  1:570972/1‑138 (MQ=255)                                                                     aaCGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGa                                                               <  1:865548/139‑1 (MQ=255)                                                                     aaCGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGa                                                               <  1:31132/139‑1 (MQ=255)                                                                                 cGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATact                                                   <  1:16387/139‑1 (MQ=255)                                                                                              acgcaaTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTc                                      <  2:129375/139‑1 (MQ=255)                                                                                                                          ggCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCa          >  2:848539/1‑139 (MQ=255)                                                                                                                             aacaaTCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCAtct       >  2:804614/1‑139 (MQ=255)                                                                                                                                 aTCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCAtcttctt   >  1:158154/1‑139 (MQ=255)                                                                                                                                  tCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCAtcttcttc  >  2:594092/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      CACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTC  >  NZ_CP009273/1810855‑1811122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑