Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	209,618	A→C	A267A (GCA→GCC)	tilS →	tRNA lysidine(34) synthetase TilS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	209,618	0	A	C	100.0%	151.3 / NA	43	A267A (GCA→GCC)	tilS	tRNA lysidine(34) synthetase TilS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/25);  total (18/25)

GCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATT  >  NZ_CP009273/209483‑209747                                                                                                                                        |                                                                                                                                  gctgGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGcccc                                                                                                                                 >  2:790215/1‑138 (MQ=255) gctgGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGcccc                                                                                                                                 >  2:164967/1‑138 (MQ=255)  ctgGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCg                                                                                                                                <  2:712649/138‑1 (MQ=255)  ctgGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGa                                                                                                                               <  1:193802/139‑1 (MQ=255)  ctgGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGa                                                                                                                               <  1:705822/139‑1 (MQ=255)       tGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCt                                                                                                                          >  2:73039/1‑139 (MQ=255)          aCTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTcc                                                                                                                       >  2:593467/1‑139 (MQ=255)                 gCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGAcg                                                                                                                <  2:113443/139‑1 (MQ=255)                   aGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGAcgcg                                                                                                              <  1:500491/139‑1 (MQ=255)                    gatgatTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGt                                                                                                             <  1:406343/139‑1 (MQ=255)                              cacaCTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGAt                                                                                                   >  1:632052/1‑139 (MQ=255)                                   tGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGAt                                                                                                   >  1:850533/1‑134 (MQ=255)                                   tGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGAt                                                                                                   <  2:850533/134‑1 (MQ=255)                                   tGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTgg                                                                                               >  2:704547/1‑138 (MQ=255)                                   tGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTgg                                                                                               >  1:777123/1‑138 (MQ=255)                                         tCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAg                                                                                        <  1:732894/139‑1 (MQ=255)                                             cgcAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGAcgcg                                                                                                              >  2:535379/1‑113 (MQ=255)                                             cgcAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGAcgcg                                                                                                              <  1:535379/113‑1 (MQ=255)                                              gcAGGGGACGCTGCAGATTGTGCCAAGGCTGGCGATGAGTGAGGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtggcg                                                                                   <  2:250676/139‑1 (MQ=255)                                               cAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtggcgc                                                                                  >  2:512112/1‑139 (MQ=255)                                                   ggACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtggcgctggc                                                                              <  1:704547/139‑1 (MQ=255)                                                     aCGCTGCAGATTGTGCCAATGCTGGCGATGAGGGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtggcgctggcgc                                                                            <  2:618652/139‑1 (MQ=255)                                                      cGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGcgcg                                                                           <  1:462299/139‑1 (MQ=255)                                                           cAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAg                                                                      <  1:164967/139‑1 (MQ=255)                                                           cAGATTGTGCCAAGGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGa                                                                        <  1:740317/137‑1 (MQ=255)                                                                   gCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCa                                                              >  1:592025/1‑139 (MQ=255)                                                                     cAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCAcc                                                            <  1:411229/139‑1 (MQ=255)                                                                     cAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCAcc                                                            <  1:553901/139‑1 (MQ=255)                                                                      aaTGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCAccc                                                           <  2:845537/139‑1 (MQ=255)                                                                        tGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTg                                                         >  2:286586/1‑139 (MQ=255)                                                                            ggCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTa                                                     >  2:734774/1‑139 (MQ=255)                                                                                   aGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTgg                                              >  1:685524/1‑139 (MQ=255)                                                                                     tGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGt                                                                                                             <  2:262191/74‑1 (MQ=255)                                                                                     tGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGt                                                                                                             >  1:262191/1‑74 (MQ=255)                                                                                       aTGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCATAATGCCCCGATGCCTTCCCGCGACGCGTTGGt                                                                                                         <  1:747641/76‑1 (MQ=255)                                                                                       aTGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGt                                                                                                         >  2:747641/1‑76 (MQ=255)                                                                                       aTGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGgcg                                           <  1:286586/138‑1 (MQ=255)                                                                                                           aTCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTAt                       >  2:564037/1‑138 (MQ=255)                                                                                                                ccgcTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAg                                                                      <  1:577770/86‑1 (MQ=255)                                                                                                                ccgcTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAg                                                                      >  2:577770/1‑86 (MQ=255)                                                                                                                 cgcTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCg                 >  1:104242/1‑138 (MQ=255)                                                                                                                          gcagggcagAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTgtgg        <  1:116013/138‑1 (MQ=255)                                                                                                                             gggcagAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGa    <  2:777123/139‑1 (MQ=255)                                                                                                                               gcagAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGAtt  <  2:461301/139‑1 (MQ=255)                                                                                                                                cagAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGAtt  >  2:744144/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                                  GCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATT  >  NZ_CP009273/209483‑209747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑