Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,834,793	A→G	D357G (GAT→GGT)	ynjE →	thiosulfate sulfurtransferase YnjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,834,793	0	A	G	100.0%	71.6 / NA	21	D357G (GAT→GGT)	ynjE	thiosulfate sulfurtransferase YnjE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/9);  total (12/9)

AATTTATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGATGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGCGCCA  >  NZ_CP009273/1834676‑1834924                                                                                                                      |                                                                                                                                    aaTTTATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCga                                                                                                                >  1:221952/1‑139 (MQ=255) aaTTTATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCga                                                                                                                <  1:273370/139‑1 (MQ=255)     tATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCgatga                                                                                                             <  2:221952/138‑1 (MQ=255)              gACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCt                                                                                                   <  2:77886/139‑1 (MQ=255)                                          aaaGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAAc                                                                          >  1:255175/1‑136 (MQ=255)                                          aaaGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAAc                                                                          >  2:853014/1‑136 (MQ=255)                                          aaaGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAAc                                                                          >  1:494304/1‑136 (MQ=255)                                                   aTAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAg                                                              <  1:287694/139‑1 (MQ=255)                                                                gTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTg                                                 >  1:536427/1‑139 (MQ=255)                                                                      gACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCAc                                           <  2:494304/139‑1 (MQ=255)                                                                        cacGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCg                                         >  2:121827/1‑139 (MQ=255)                                                                          cGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGc                                       >  2:55267/1‑139 (MQ=255)                                                                           gCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTg                                                                                                                                   >  1:387906/1‑46 (MQ=255)                                                                           gCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTg                                                                                                                                   <  2:387906/46‑1 (MQ=255)                                                                                     aCTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTc                            >  2:473645/1‑139 (MQ=255)                                                                                       tCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTcc                           >  2:811068/1‑138 (MQ=255)                                                                                         gACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGa                         <  1:811068/138‑1 (MQ=255)                                                                                         gACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGa                         <  2:30680/138‑1 (MQ=255)                                                                                         gACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGa                         >  1:383458/1‑138 (MQ=255)                                                                                                          ttCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTacgcacg       >  1:98038/1‑139 (MQ=255)                                                                                                                aCCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGCGCCa  <  2:294930/138‑1 (MQ=255)                                                                                                                      |                                                                                                                                    AATTTATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGATGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGCGCCA  >  NZ_CP009273/1834676‑1834924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑